TCR Design Construction and Plasmid GLP Production

As a leading provider of customized services, Creative Biolabs devotes to the development of T cell therapy, such as T cell receptor (TCR) gene therapy. Through altering the specificity of T cell receptors (TCR), T cells can be genetically modified to enhance their tumor-killing activity, which is considered as a fascinating approach. Undoubtedly, Creative Biolabs offers products and services for T cells modified by TCR.

Fig.1 TCR recognizes the tumor.

Affinity Enhanced TCR Service

CAR-T technology, through a series of cooperative procedures, finally meets the requirements of FDA for cell therapy (Fig.2). It is also imperative for us to provide high-affinity TCR, and increase the sensitivity and specificity of antigen recognition. High-affinity TCR can be achieved by Creative Biolabs in several ways:

• Antigen peptide stimulates allogeneic reactive T cells to produce peptide-specific TCR;
• Antigen peptide immunizes HLA transgenic mice to obtain murine TCR;
• In vitro mutates TCR against complementarity determining region 3 (CDR3), the mutant TCR has a higher antigenic peptide. MHC binding characteristics;
• Use of codon to optimize TCR;
• To clear N-glycosylation sites in the TCR alpha, beta chain c region.

Fig.2 Identification of T-cell receptors with desired specificity and optimal affinity.

TCR Gene Transduction System Service

Creative Biolabs is a leader in the field of genetically engineered T cells for cancer treatment, providing gene transduction system service for the generation of TCR-modified T cells. With our advanced technology and rich experience, we provide the most professional carrier service for the cutting-edge transgenic immunotherapy.

Fig.3 Manufacturing and delivery pipeline of genetically modified T-cell therapies

• Virus vector system
  Retrovirus vectors and lentivirus vectors are utilized to facilitate the stable and efficient expression of the target gene in the target cells, which shows high efficiency of transduction.
• Non-virus vector system
  The vector offers transposons, Sleeping Beauty and PiggyBac, they provide potential for convenient and quick research for clinical application in the later stage.

Our GLP Plasmid Production Service

Based on the general approach of TCR construction, Creative Biolabs provides an optimized TCR carrier production method.

• The one-step method greatly simplifies the production process of TCR vectors
• The traditional three-step method usually involves cloning the entire TCR gene, producing TCR expression boxes and constructing the structure of retroviruses.
• We launch the GLP-grade plasmid preparation service. Each experimental step in the plasmid preparation service has been standardized.

Highlights

• Accumulate rich experience in plasmid DNA preparation.
• Cover monomer construction in research laboratories, large-scale production by pharmaceutical companies, and the provision of high-purity and endotoxin-free plasmids.
• Provide DNA plasmids for gene therapy studies under GLP standards.
• Able to customize the design and structure of TCR products for customers according to their special needs.

Creative Biolabs scientists are committed to being a reliable partner of your TCR design construction and GLP plasmid production and providing the most efficient solution for your project. Tell us what you need and we will provide a guide to project cost estimates and timelines.

Please contact us and our team will get back to you as soon as possible.

References

1. Heslop, H. E. Genetic engineering of T-cell receptors: TCR takes to titin[J]. Blood. 2013, 122(6): 853-854.
2. Karpanen T, Olweus J. T-cell receptor gene therapy – ready to go viral[J]. Molecular Oncology. 2015, 9(10): 2019-2042.
3. Sharpe M, Mount N. Genetically modified T cells in cancer therapy: opportunities and challenges[J]. Disease Models & Mechanisms. 2015, 8(4): 337-350.

For Research Use Only | Not For Clinical Use