Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsThe Phage Display HuVHHL-S2 Humanized Semi-synthetic Single Domain Antibody Library is a cutting-edge resource for high-throughput antibody discovery. Our platform allows you to speed up drug discovery through unmatched diversity and precision while offering customizable solutions powered by sdAbs. Our library provides consistently high-affinity candidates that meet your specific needs when you are investigating new diagnostics or innovative therapeutic solutions.
| Library Type | Humanized Library | Library ID | HuVHHL-S2 |
| Display Technology | M13 pIII fusion, Phagemid phage display system | Library Format | Humanized Semi-synthetic VHH |
| Vector System | pCantab 5E | Tags | E tag |
| Library Capacity | >1.0×1010 | Randomization | Synthetic trimer-codon HCDR3 |
| Host Strain |
TG1 cells for proliferation HB2151 cells for antibody production |
Helper Phage | M13KO7 |
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Humanization Design Humanizing VHH fragments enhances their clinical application potential by reducing immunogenic responses, which are often a challenge in therapeutic antibody development. This process ensures that the antibodies are better tolerated in human subjects, increasing their effectiveness and safety in medical applications. |
Exceptional Diversity and Synthetic Precision The HuVHHL-S2 library delivers exceptional synthetic diversity which leads to a wide array of potential antibody candidates. The semi-synthetic framework removes the inherent biases that occur in conventional animal-based antibody libraries to guarantee consistent and reproducible results. The novel design ensures your research maintains high reliability and consistently produces valuable results. |
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Compact Size, Big Potential SdAbs have a much smaller size compared to classic antibodies which results in superior tissue penetration and the capacity to target intracellular epitopes. Their small dimension enables these molecules to access areas that were once unreachable like deep tumor regions or specific cell compartments. SdAbs become prime subjects for pioneering medical treatments because they enable the creation of bispecific antibodies and cell-internalizing antibodies which expand beyond traditional antibody capabilities. |
Unmatched Affinity and Specificity SdAbs demonstrate exceptional binding affinity and specificity that allows them to efficiently target small proteins and low molecular weight compounds. The special structural characteristics of sdAbs enable them to reach hard-to-access epitopes which allows precise antigen binding with exceptional accuracy. Their structure makes single-domain antibodies excellent choices for use in drug delivery systems and therapeutic applications. |
Creative Biolabs' HuVHHL-S2 sdAb Library offers scalable, high-efficiency phage display screening to accelerate the discovery process across a wide range of target classes. Our streamlined approach eliminates the complexities of library management and experimental analysis, allowing you to focus on data interpretation and subsequent applications. By combining cutting-edge phage display technology with expert assay design, we enable the rapid identification of high affinity sdAbs. These antibodies are ideal for advanced research, as well as diagnostic and therapeutic applications, ensuring that your projects progress faster and more effectively.
The HuVHHL-S2 library supports screening against a wide range of target types, including:
| Specialized Antibody Formats | |||
| Bispecific Antibodies | Anti-Idiotype Antibodies | Anti-Epitope Antibodies | Cell-Internalizing Antibodies |
| Broad Range of Target Types | |||
| Membrane Protein Targets | Cell-based Targets | Enzyme Inhibitors | MHC-Peptide Complexes |
| Disease and Biomarker Targets: | Pathogen-derived Antigens | Cancer Antigens | Receptor Targets |
| Small Molecule Targets | Toxin Targets | ||
Our high-efficiency phage display screening follows a rigorous multi-round biopanning approach that ensures optimal antibody selection. From antigen submission to post-screening support, the process is designed to streamline and accelerate antibody discovery:
Provide antigen details, including recombinant proteins, peptides, cells, or small molecules.
We perform biopanning under optimized conditions using the HuVHHL-S2 library to identify potential high-affinity binders.
You receive sequence data, binding results (e.g., ELISA, SPR), and clone information.
Our expert team supports candidate validation and further application development, ensuring the antibodies are ready for advanced research or clinical use.
Note: We offer screening-only services and do not provide enriched phage pools post-panning. This ensures a clear focus on delivering quality-controlled, sequence-defined antibody candidates optimized for downstream applications.
| Case: Library Screening Against Cell-Based Targets | |
| Objective | To identify high-affinity sdAbs targeting membrane proteins overexpressed on cell lines, utilizing the HuVHHL-S2 phage display library. |
| Starting Materials |
Target: Membrane proteins overexpressed on cell lines (target protein T1). Control: Control protein and cell line background controls to account for non-specific binding. |
| Cell-based Screening Strategy |
Pre-counter Selection: To eliminate phages that bind non-specifically to the cell lines or the control proteins, ensuring the selected clones specifically target the overexpressed membrane protein. Cell-Based Biopanning: Perform multiple rounds of biopanning to enrich for phages that bind to the overexpressed target membrane protein while minimizing binding to control protein and background cell lines. Binder Validation: The enriched phages are tested using flow cytometry and cell-based ELISA to assess their binding affinity and specificity to the target protein on live cells. |
| Key Results |
  After several rounds of biopanning and screening, multiple clones were identified with high binding affinity and specificity for the target membrane protein overexpressed in the cell lines. These clones demonstrated minimal binding to the control protein or background cell lines. The successful clones were further validated through phage ELISA and FACS, confirming their ability to specifically recognize and bind to the membrane protein on living cells. |
Creative Biolabs offers a vast range of premade sdAb libraries sourced from multiple species such as camel, alpaca, llama alongside humanized sdAbs. Researchers have access to these libraries in synthetic and natural formats which enables their use for studies from target discovery to advanced therapeutic development. Our unique screening services enable precise and reliable identification of high-affinity binding molecules. Research teams looking to enhance their flexibility can obtain licenses to specific libraries for easy integration into their scientific studies and product development processes.
| Antibody Library ID | Display Technology | Library Format | Library Size | Out-licensing |
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HuVHHL-S1 ⭐️ Premium Selection |
pIII-fusion, Phagemid Phage Display | Synthetic VHH | >1.0×1011 | Screening-only |
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HuVHHL-S2 ⭐️ Premium Selection |
pIII-fusion, Phagemid Phage Display | Semi-synthetic VHH | >1.0×1010 | Screening-only |
Creative Biolabs focuses on delivering customizable phage display libraries with high-quality standards to address your research requirements. Our deep antibody discovery experience provides complete support throughout the process of library screening to post-screening validation.
Reach out today to learn how our specialized libraries and advanced screening platforms can accelerate your research and bring your goals to life. Let’s work together to find the perfect solution tailored to your needs!
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
