Anti-Cell-Based Target Antibody Discovery from Premade Phage Displayed Antibody Library

Creative Biolabs possesses enriched experience in antibody production and manufacture, performing various strategies to offer customized antibodies. For recent years, scientists from Creative Biolabs are devoted to helping global researchers discover specific antibodies with our premade phage displayed antibody libraries. Based on our advanced technologies and well-established platform, we aim to help you discover high-affinity anti-cell-based target antibody to accelerate your programs.

Anti-Cell-Based Target Antibody

Cell surface antigens (usually membrane protein and cell surface receptors) are attractive targets for antibody production both for the isolation of rare cell populations and therapeutic targeting. However, most of these antigens are not available in quantities sufficient for immunization unless generated artificially by recombinant gene expression. Furthermore, expressing these membrane proteins in large amounts is difficult and, outside of the hydrophobic environment of the cell membrane, these proteins can undergo conformational changes and/or aggregation. This means they become structurally different from what exists in nature, which consequently might lead to isolating binders that do not identify the antigen in its native form. Therefore, a novel approach should be developed for the production of anti-cell-based target antibodies.

Anti-Cell-Based Target Antibody Discovery from Premade Phage Displayed Antibody Library Fig.1 Whole cell-based panning using Biopanning and Rapid Analysis of Selective Interactive Ligands (BRASIL). (Alfaleh, 2017)

Premade Phage Displayed Antibody Library Screening for Anti-Cell-Based Target Antibody

Recently, screening antibody libraries against cell surface antigens, such as membrane proteins, in their native or even near-native conformations using whole-cells has been applied for the anti-cell-based target antibody production. Compared to the traditional antibody preparation method, whole-cell screening enables to retrieve antibody clones that are specific for target antigens that are present on the surface of specific cell populations. Also, it can isolate antibodies with more biologically relevant antibodies that depend on binding to a specific antigenic determinant that is naturally exposed to the targeted protein. With years of experience, Creative Biolabs has established various phage displayed antibody libraries with large capacity and diversity. Our professional scientists will help you screen high-affinity anti-cell-based target antibodies with affinity ranging from pM to nM. The premade phage displayed antibody libraries available in Creative Biolabs are listed as following:

Creative Biolabs also provides antibody discovery services for different types of targets to suit a variety of applications:

Advantages of Our Premade Library Screening Service

Highly efficient and robust library resources with large capacity and diversity
Particularly suitable for antigens that are difficult to prepare, or antigens that cannot elicit immune responses in animals
Comprehensive services from project design to data interpretation
Professional scientists that work closely with customers to tailor the most efficient and reliable service

Over the years, Creative Biolabs has been dedicated to providing world-class premade phage antibody library screening services to global customers from both industries and academies. Based on our well-established phage antibody library screening platform, we have successfully screened numerous high specific, high-affinity antibody candidates targeting various antigens. For more detailed information, please feel free to contact us or directly send us a quote.

Reference

Alfaleh, M.A., et al. (2017). Strategies for selecting membrane protein-specific antibodies using phage display with cell-based panning. Antibodies, 6(3), p.10.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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