Custom ssRNA Synthesis Service
Introduction
Creative Biolabs' Custom ssRNA Synthesis service accelerates drug discovery and research via advanced chemical synthesis, delivering high-purity, sequence-verified ssRNA molecules. Tailored to specifications for gene expression, RNAi, vaccine development, and antiviral studies, it overcomes yield, purity, and modified RNA synthesis challenges, ensuring efficient project progression with reliable, consistent outputs.
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Custom ssRNA Synthesis
Single-stranded RNA (ssRNA) molecules are pivotal in modern molecular biology and biotechnology, serving as genetic material in many viruses, crucial regulators of gene expression, and versatile tools in research and therapeutics. From enabling the groundbreaking development of mRNA vaccines to facilitating advanced gene editing and antiviral strategies, the ability to precisely synthesize custom ssRNA is fundamental.
Single-stranded RNA (ssRNA) is a type of RNA molecule that does not form a double-stranded structure. According to its function, it can be classified as:
- mRNA (messenger RNA): It carries genetic information to guide protein synthesis, has A cap structure at the 5' end (m⁷GpppN), and a poly (A) tail at the 3' end.
- Non-coding ssRNAs, including tRNA, rRNA, siRNA, miRNA, lncRNA, circRNA, etc., are involved in gene expression regulation, RNA processing, etc.
- Virus (-ssRNA), their single genomic strands directly participate in replication or transcription.
The structural feature is that it relies on base complementarity to form secondary structures such as stem-loops, which affect functions (such as ribosome binding sites).
It is easily degraded by RNase and requires chemical modification (such as 2'-O-methyl, lock nucleic acid LNA) to enhance stability.
Workflow
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Required Starting Materials
- Target RNA Sequence: The precise nucleotide sequence (5' to 3') of the ssRNA molecule you require.
- Desired Modifications: A clear list of any chemical modifications and their specific positions within the sequence.
- Purity and Scale Requirements: The desired purity level and the quantity of RNA needed (e.g., nanomoles, micromoles).
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Synthesis
We synthesize custom ssRNA via a controlled chemical process, conjugating activated ribonucleotides to solid-supported chains for sequence fidelity. Using proprietary tech, we incorporate modifications (bases, phosphorothioate backbones, 5' caps, 3' poly(A) tails) to precisely regulate RNA properties for viral research and RNA therapeutics.
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Purification
Post-synthesis, crude ssRNA contains full-length product, truncations, and impurities. Rigorous purification via RNase-free PAGE/HPLC in RNase-free environments ensures high quality for sensitive applications, preventing RNA degradation.
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Quality Control
- MALDI-TOF Mass Spectrometry (MS): Verifies ssRNA molecular weight to confirm correct sequence and modification incorporation.
- High-Performance Liquid Chromatography (HPLC): Assesses purity by quantifying full-length product ratio against impurities.
- Capillary Gel Electrophoresis (CGE): Provides high-resolution size-based separation for product integrity and homogeneity. Rigorous QC ensures every ssRNA meets the highest research and development standards.
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Final Formulation & Packaging
The purified and quality-controlled ssRNA is formulated according to your specifications (e.g., lyophilized or in solution) and packaged in RNase-free tubes for safe and stable delivery.
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Estimated Timeframe
The typical timeframe for this service ranges from 1 to 3 weeks, depending on the complexity of the sequence, the number and type of modifications, and the required purity level.
What We Can Offer
- Tailored Synthesis Solutions: Flexible design for custom ssRNA sequences, lengths, and chemical modifications to meet project specs.
- Advanced Synthesis Technology: Proprietary platforms ensure high coupling efficiency, sequence integrity, and seamless complex modification incorporation.
- Rigorous Quality Assurance: MALDI-TOF, HPLC, and CGE validate molecular weight, purity, and integrity for reliable results.
- Scalable Production Capabilities: Microgram to gram-scale synthesis for research, preclinical, and therapeutic needs.
- Expert Scientific Consultation: RNA specialists guide sequence design, modification selection, and application optimization.
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Customer Reviews
FAQs
How to reduce dsRNA impurities when synthesizing ssRNA through in vitro transcription (IVT)?
Several methods can be adopted to optimize the production process. For instance, when designing the template, a single-strand protruding sequence downstream of the T7 promoter (such as adding six uracil at the 5' end) can be inserted to reduce the annealing of transcription products. During the reaction, optimize the reaction conditions, increase the magnesium ion concentration (10-15 mM) to stabilize the single-strand structure, control the transcription temperature (37°C→42°C) to inhibit the formation of double-strands, and finally enhance the purification process by using DsrNA-specific enzymes (such as RNase III) to digest impurities. Single chains were separated by anion exchange chromatography (Q Sepharose).
How to optimize the delivery efficiency of ssRNA in primary cells?
Cell type-specific strategy
- Immune cells: ssRNA is modified with TLR7/8 agonists, and endocytosis is mediated by innate immune receptors.
- Neuron cells: Encapsulated with ionizable lipid nanoparticles (LNP), the formula incorporates neuro-targeted peptides, enhancing the efficiency of crossing the blood-brain barrier by 20 times.
- Plant cells: Through Agrobacterium-mediated transformation, complexes are formed to enhance cells and delivery
How to quantitatively detect the subcellular distribution of ssRNA within cells?
High-resolution positioning solution
- RNA-FISH: Design fluorescently labeled probes and combine confocal microscopy to distinguish nuclear/plasmic distribution (resolution ~200 nm).
- Mass flow cytometry: couple ssRNA with metal isotope-labeled antibodies to simultaneously analyze RNA expression and cell surface markers.
- Photocross-linking sequencing (CLIP-seq): UV-induced ssRNA-protein cross-linking, followed by sequencing after immunoprecipitation to locate binding sites (such as the interaction regions between Ago2 and miRNA).
Creative Biolabs' Custom ssRNA Synthesis service provides high-quality, precisely engineered single-stranded RNA molecules, essential for advancing your research in therapeutics, diagnostics, and fundamental biology. Leveraging advanced synthesis technologies and stringent quality control, we empower you to overcome the complexities of RNA production, accelerating your path to discovery and development.
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