Plain Liposome

Product Details

Creative Biolabs offers a wide range of high-quality plain liposome products in different formulations. All the products are prepared by our advanced LipoDrive™ technology platform using the highest quality lipids provided by leading global ingredient suppliers. The particle size of liposomes is ranged from 50nm to micron level with high batch-to-batch consistency and homogeneity. You can choose any of the formulations, PEGylated or non-PEGylated according to your experimental needs.

Liposomes have received extensive attention and are widely investigated as a promising drug carrier. They can be functionalized through the incorporation of different small molecules, surface modifications, and valent coupling. Plain liposomes have the simplest lipid composition and function and therefore be used to study lipid membrane-protein interactions, in vivo studies of liposomes, and encapsulation of hydrophobic drugs. Liposomes made from phosphatidylserine (PS) are negatively charged vesicles, which can be used to mimic cell membranes. Positively or neutrally charged liposomes are also available, mainly consisting of DOTAP and Phosphatidylcholine (PC) respectively. Additionally, all the plain liposome products can be loaded with hydrophobic drugs through hydrophobic interaction or used as control formulations for drug-loaded liposomes. If you are looking for any other liposome products, please see our liposome development service, every parameter of the liposome is highly customizable.

As a leader in the field of liposome development, Creative Biolabs has served clients all over the world. Each batch of our product is freshly manufactured with the best quality and stability. Feel free to contact us for any technical support about our products.

For Research Use Only. Not For Clinical Use

Technical Note

1. Liposomes are typically unilamellar structures measuring around 100nm but can be customized to sizes between 50nm and 200nm, or even larger. Liposomes exceeding 200nm develop a multilamellar structure.
2. Liposomes are usually synthesized in a phosphate-buffered saline (PBS) buffer at pH 7.4, but can be adapted using alternative buffers based on specific requirements.
3. Unsaturated lipids such as DOPG, DOPC, and DOTAP that have 'kinky' fatty acid tails form loose packs in liposomes, influencing their packing and surface interaction. This is in contrast to saturated lipids which create tightly packed areas.
4. It's important to note that natural phosphatidylcholine (PC) extracts used in liposome composition are a mix of saturated and unsaturated lipids. The lipid composition varies with different PC extracts. For example, soy-derived PC extracts are predominantly unsaturated, whereas extracts from eggs, liver, or brain contain roughly equal proportions of both lipids.
5. During liposome production, sterility is preserved under a sterile hood in a degassed buffer, which is purged with argon to prevent unsaturated phospholipid oxidation. This process, alongside their small size, ensures that liposomes remain dispersed in the suspension, avoiding sedimentation at the vial bottom.
6. Storage of liposomes must meet two important conditions. They should be stored at 4°C to circumvent potential hydrolysis and must never be frozen as the formation of ice crystals can rupture the lipid membranes, leading to an alteration in their size.

Publish Data

In situ modification of plain liposomes with lipidated coiled coil forming peptides induces membrane fusion
Journal of the American Chemical Society
Author: Versluis, F., Voskuhl, J., et al.

The graph illustrates the viability of HEK 293 cells treated with various concentrations of f-MWCNT, CLC (CNT-liposome conjugate), and plain liposomes. Plain liposomes exhibit relatively low toxicity to the cells, while f-MWCNTs show higher toxicity even at lower concentrations. The CLC displays similar toxicity levels to f-MWCNT, suggesting that the presence of liposomes does not significantly alter the toxicity profile. This highlights the importance of plain liposomes as a control, providing a baseline for comparing the effects of f-MWCNT and CLC on cell viability.

Fig. 1 HEK 293 cell viability at different f-MWCNT, CLC and plain liposomes concentrations

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For Research Use Only. Not For Clinical Use