Cationic Liposome Development Service

Cationic liposomes are one of the most commonly used non viral gene vectors, and are widely used as standard gene vectors for in vitro transfection. They have been widely used because of their low toxicity, low immunogenicity, high drug loading capacity and convenient preparation.

Background of Cationic Liposome

In gene delivery, non-viral vectors have become the preferred carrier system for DNA delivery. They can overcome major viral problems, such as immunogenicity and mutagenicity. Cationic lipid-mediated gene transfer is one of the most commonly used non-viral vectors and has been shown to be a safe and effective vector. Cationic liposome is a structure made of positively charged lipids. Because of their good interaction with negatively charged DNA and cell membranes, they are increasingly being studied for gene therapy. They have demonstrated their selectivity to tumor vascular endothelial cells, which brings hope to anti-angiogenesis and anti-vascular treatments. When administered intravenously, they can also be selectively delivered to the lung and liver.

Fig.1 Proposed mechanisms of cationic liposome condensation and uptake

Strategy of Cationic Liposome Formulation

Cationic liposomes are produced using lipids that can induce a positive charge on the surface of liposomes, for example, 1, 2-oleinyl-3-trimethylammonium-propane (DOTAP), N- [1 -(2,3-oleoyloxy)propyl] -N,N,N-trimethyl-ammonium methyl sulfate, dioleoylphosphatidyl ethanolamine (DOPE), oleic acid (OA) and dimethyldioctadecyl ammonium bromide. After interacting with negatively charged DNA, cationic liposomes form clusters of aggregated vesicles. At a critical density, DNA will condense and be encapsulated in a lipid bilayer, although liposomes may bind along the DNA surface and maintain its shape. Compared with classical liposomes, they can also interact with negatively charged cell membranes more easily. The fusion between cationic vesicles and the cell surface can directly deliver DNA through the plasma membrane. This process bypasses the endosome-lysosome pathway, which leads to the degradation of anionic liposome formulations.

What We Can Do about Cationic Liposome Development?

Creative biolabs has rich experience in the development of various functional liposomes. We can not only complete custom lipid synthesis and characterization technology, but also combine it with gene expression and silencing analysis to reveal the relationship between structure and function in vitro. If you are interested in our service, please feel free to contact us.

For Research Use Only. Not For Clinical Use