Plain Liposome Products

Product Details

Creative Biolabs offers a wide range of high-quality plain liposome products in different formulations. All the products are prepared by our advanced LipoDrive™ technology platform using the highest quality lipids provided by leading global ingredient suppliers. The particle size of liposomes is ranged from 50nm to micron level with high batch-to-batch consistency and homogeneity. You can choose any of the formulations, PEGylated or non-PEGylated according to your experimental needs.

Liposomes have received extensive attention and are widely investigated as a promising drug carrier. They can be functionalized through the incorporation of different small molecules, surface modifications, and valent coupling. Plain liposomes have the simplest lipid composition and function and therefore be used to study lipid membrane-protein interactions, in vivo studies of liposomes, and encapsulation of hydrophobic drugs. Liposomes made from phosphatidylserine (PS) are negatively charged vesicles, which can be used to mimic cell membranes. Positively or neutrally charged liposomes are also available, mainly consisting of DOTAP and Phosphatidylcholine (PC) respectively. Additionally, all the plain liposome products can be loaded with hydrophobic drugs through hydrophobic interaction or used as control formulations for drug-loaded liposomes. If you are looking for any other liposome products, please see our liposome development service, every parameter of the liposome is highly customizable.

As a leader in the field of liposome development, Creative Biolabs has served clients all over the world. Each batch of our product is freshly manufactured with the best quality and stability. Feel free to contact us for any technical support about our products.

For Research Use Only. Not For Clinical Use

Technical Note

1. Liposomes are typically unilamellar structures measuring around 100nm but can be customized to sizes between 50nm and 200nm, or even larger. Liposomes exceeding 200nm develop a multilamellar structure.
2. Liposomes are usually synthesized in a phosphate-buffered saline (PBS) buffer at pH 7.4, but can be adapted using alternative buffers based on specific requirements.
3. Unsaturated lipids such as DOPG, DOPC, and DOTAP that have 'kinky' fatty acid tails form loose packs in liposomes, influencing their packing and surface interaction. This is in contrast to saturated lipids which create tightly packed areas.
4. It's important to note that natural phosphatidylcholine (PC) extracts used in liposome composition are a mix of saturated and unsaturated lipids. The lipid composition varies with different PC extracts. For example, soy-derived PC extracts are predominantly unsaturated, whereas extracts from eggs, liver, or brain contain roughly equal proportions of both lipids.
5. During liposome production, sterility is preserved under a sterile hood in a degassed buffer, which is purged with argon to prevent unsaturated phospholipid oxidation. This process, alongside their small size, ensures that liposomes remain dispersed in the suspension, avoiding sedimentation at the vial bottom.
6. Storage of liposomes must meet two important conditions. They should be stored at 4°C to circumvent potential hydrolysis and must never be frozen as the formation of ice crystals can rupture the lipid membranes, leading to an alteration in their size.

Publish Data

Protective effect of saccharides on freeze-dried liposomes encapsulating drug
Author: Guimarães, Diana, et al.

This study investigated the protective effects of adding different saccharides to liposomes during the freeze-drying process. Researchers added five saccharides at varying concentrations to the liposomes: trehalose, lactose, glucose, mannitol, and sucrose. Subsequently, the size and polymer dispersity index (PDI) of liposomes after the freeze-drying-rehydration cycle were measured to monitor the aggregation and fusion of liposomes in each group during the freeze-drying process. The results showed that, compared to plain liposomes, liposomes with saccharides could prevent aggregation and fusion in a concentration-dependent manner, thereby maintaining the integrity of freeze-dried liposomes. Among them, the protective effect of sucrose was most significant, making it a promising method for liposome storage. This highlights the importance of plain liposomes as controls, providing a baseline for studying the protective effect of saccharides as cryoprotectants on liposomes.

Fig.1 The particle size and PDI of each group of liposomes.^1,2

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For Research Use Only. Not For Clinical Use