Fluorescent Liposome Products

Product Details

Creative Biolabs offers various types of fluorescent liposomes labeled with lipophilic dyes for different applications. Our fluorescent liposome products include different sizes from 100 nm series to 500 nm to satisfy the need for particle size standards in the field of flow cytometry. The particle size present in the product sheet is for the mean diameter as measured by a standard DLS particle sizer.

Fluorescent liposomes are a new type of fluorescent markers by unilamellar liposomes encapsulated and surface-immobilized fluorophores to image flow profiles in microfabricated structures. The liposomes were produced with phospholipids and cholesterol by extrusion through a polycarbonate membrane. Carboxyfluorescein in the aqueous core and fluorescein labeled lipids in the bilayer endow them surface and volume fluorescence to maximize their fluorescence intensity. The composition of the liposome was chosen to give the liposome a net negative charge to minimize self-aggregation and interaction with the negatively charged channel glass surface. These liposomes were monodisperse, neutrally buoyant, and hydrophilic and exhibited no adsorption on glass surfaces.

Our fluorescent liposomes can be used to investigate pressure-driven flow and provide images with excellent signal-to-noise ratio. Fluorescent liposomes also can be custom-made for various applications to offer a broad range of surface and volume characteristics such as charge, size, and surface chemistry.

For Research Use Only. Not For Clinical Use

Technical Note

1. The primary concern with fluorescent clodronate liposomes or mannosylated fluorescent liposomes involves the potential generation of inaccurate or uninterpretable data. This is due to the disruption of the fluorescent lipid during clodronate liposomes-induced macrophage apoptosis causing it to disperse among subsequent phagocytosing macrophages. High background fluorescence may also result from lingering fluorescent lipids in undisposed extracellular "garbage". The time of apoptosis onset with clodronate liposome treatment can vary widely, hence for the most accurate results, tracking should be established within each experimental model. For tracking purposes, Creative Biolabs may make DiA/DiD/DiI/DiR-labelled clodronate liposomes available upon request which can provide clear and valid biodistribution data.
2. Due to margination post-liposome phagocytosis, circulating monocytes may "disappear" or show reduced counts within the first 2 hours. Such cells usually return to the circulation within a few hours.
3. For in vivo injections, liposomes should be homogeneously suspended. To maintain homogeneity, slowly invert the vial prior to usage and avoid vigorous shaking to prevent foaming.
4. If unacquainted with large-volume injections, have extra animals on hand for practice to minimize injection-related adverse events.
5. For intravenous dosing, adopt standard precautions for larger volumes, including warming the product to room temperature, ensuring air bubble removal, and injecting at a steady rate. If unusual animal reactions are observed, the infusion rate should be reduced.
6. Animals may exhibit gasping or seizures post-infusion, however, most tend to recover without permanent injury.
7. Liposomes should be stored at 4°C and freezing should be avoided.

Publish Data

Improved vessel painting with carbocyanine dye-liposome solution for visualisation of vasculature
Author: Konno, Alu, Naoya Matsumoto, and Shigetoshi Okazaki.

This study used fluorescent liposomes for vascular visualization studies. The researchers employed DiIC18 and DiIC12 liposomes to perfuse mice's left ventricles. Figure A shows that the DilC12 liposome stated veins that were nearly invisible with DilC18 staining. Figure B illustrates how vascular endothelial cells may be identified in the DiIC12 liposome-perfused brain. Some cells' nuclei were evident even in the absence of nuclear labeling. This work shows that fluorescent liposomes (DiIC12 liposomes) are a simple, practical, and cost-effective approach for vascular visibility, expanding the options for visualization investigations.

Fig.1 Vessel painting with DiIC18 liposome and DiIC12 liposome.^1,2

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For Research Use Only. Not For Clinical Use