Creative Biolabs offers various types of fluorescent liposomes labeled with lipophilic dyes for different applications. Our fluorescent liposome products include different sizes from 100 nm series to 500 nm to satisfy the need for particle size standards in the field of flow cytometry. The particle size present in the product sheet is for the mean diameter as measured by a standard DLS particle sizer.
Fluorescent liposomes are a new type of fluorescent markers by unilamellar liposomes encapsulated and surface-immobilized fluorophores to image flow profiles in microfabricated structures. The liposomes were produced with phospholipids and cholesterol by extrusion through a polycarbonate membrane. Carboxyfluorescein in the aqueous core and fluorescein labeled lipids in the bilayer endow them surface and volume fluorescence to maximize their fluorescence intensity. The composition of the liposome was chosen to give the liposome a net negative charge to minimize self-aggregation and interaction with the negatively charged channel glass surface. These liposomes were monodisperse, neutrally buoyant, and hydrophilic and exhibited no adsorption on glass surfaces.
Our fluorescent liposomes can be used to investigate pressure-driven flow and provide images with excellent signal-to-noise ratio. Fluorescent liposomes also can be custom-made for various applications to offer a broad range of surface and volume characteristics such as charge, size, and surface chemistry.
Paramagnetic and fluorescent liposomes for target-specific imaging and therapy of tumor angiogenesis
Angiogenesis
Author: Strijkers, G. J., Kluza, E., et al.
Targeted liposomes, conjugated with RGD peptides, are used to visualize tumor angiogenesis by specifically binding to the avβ3 integrin on angiogenic tumor endothelium. In vivo MRI results reveal that RGD-conjugated liposomes accumulate predominantly in the tumor's rim, highlighting active angiogenesis. Conversely, nonspecific RAD-conjugated liposomes show a more uniform distribution throughout the tumor. The study emphasizes the critical role of fluorescent liposomes, as they enable the validation of MRI findings through ex vivo fluorescence microscopy. This microscopy confirms the specific association of RGD-conjugated liposomes with tumor blood vessels, while RAD-conjugated liposomes demonstrate nonspecific extravasation.
Fig. 1 In vivo visualization of tumor angiogenesis by application of avβ3 integrin-targeted paramagnetic and fluorescent liposomes
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Cat | Product name | Lipid Composition | Data sheet | MSDS | Inquiry |
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CLBD003LY | Fluorescent Liposome DiA | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
CLBD004LY | Fluorescent Liposome DiD | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
CLBD005LY | Fluorescent Liposome DiI | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
CLBD007LY | Fluorescent Liposome DiR | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
CLBD003LYm | Mannosylated Fluorescent Liposome DiA | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
CLBD004LYm | Mannosylated Fluorescent Liposome DiD | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
CLBD005LYm | Mannosylated Fluorescent Liposome DiI | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
CLBD007LYm | Mannosylated Fluorescent Liposome DiR | L-alpha-Phosphatidylcholine, Cholesterol | Inquiry | ||
LDLY-0123-LY209 | DiD Labeled Liposomes | HSPC: 56.2M Cholesterol: 38.5M DSPE-mPEG2000: 5.3M |
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LDLY-0123-LY210 | DiR Labeled Liposomes | HSPC: 56.2M Cholesterol: 38.5M DSPE-mPEG2000: 5.3M |
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