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Fluorescent Liposome

Product Details Technical Note Publish Data FAQs Resources

Product Details

Creative Biolabs offers various types of fluorescent liposomes labeled with lipophilic dyes for different applications. Our fluorescent liposome products include different sizes from 100 nm series to 500 nm to satisfy the need for particle size standards in the field of flow cytometry. The particle size present in the product sheet is for the mean diameter as measured by a standard DLS particle sizer.

Fluorescent liposomes are a new type of fluorescent markers by unilamellar liposomes encapsulated and surface-immobilized fluorophores to image flow profiles in microfabricated structures. The liposomes were produced with phospholipids and cholesterol by extrusion through a polycarbonate membrane. Carboxyfluorescein in the aqueous core and fluorescein labeled lipids in the bilayer endow them surface and volume fluorescence to maximize their fluorescence intensity. The composition of the liposome was chosen to give the liposome a net negative charge to minimize self-aggregation and interaction with the negatively charged channel glass surface. These liposomes were monodisperse, neutrally buoyant, and hydrophilic and exhibited no adsorption on glass surfaces.

Our fluorescent liposomes can be used to investigate pressure-driven flow and provide images with excellent signal-to-noise ratio. Fluorescent liposomes also can be custom-made for various applications to offer a broad range of surface and volume characteristics such as charge, size, and surface chemistry.

For Research Use Only. Not For Clinical Use

Technical Note

  1. The primary concern with fluorescent clodronate liposomes or mannosylated fluorescent liposomes involves the potential generation of inaccurate or uninterpretable data. This is due to the disruption of the fluorescent lipid during clodronate liposomes-induced macrophage apoptosis causing it to disperse among subsequent phagocytosing macrophages. High background fluorescence may also result from lingering fluorescent lipids in undisposed extracellular "garbage". The time of apoptosis onset with clodronate liposome treatment can vary widely, hence for the most accurate results, tracking should be established within each experimental model. For tracking purposes, Encapsula may make DiA/DiD/DiI/DiR-labelled clodronate liposomes available upon request which can provide clear and valid biodistribution data.
  2. Due to margination post-liposome phagocytosis, circulating monocytes may "disappear" or show reduced counts within the first 2 hours. Such cells usually return to the circulation within a few hours.
  3. For in vivo injections, liposomes should be homogeneously suspended. To maintain homogeneity, slowly invert the vial prior to usage and avoid vigorous shaking to prevent foaming.
  4. If unacquainted with large-volume injections, have extra animals on hand for practice to minimize injection-related adverse events.
  5. For intravenous dosing, adopt standard precautions for larger volumes, including warming the product to room temperature, ensuring air bubble removal, and injecting at a steady rate. If unusual animal reactions are observed, the infusion rate should be reduced.
  6. Animals may exhibit gasping or seizures post-infusion, however, most tend to recover without permanent injury.
  7. Liposomes should be stored at 4°C and freezing should be avoided.

Publish Data

Paramagnetic and fluorescent liposomes for target-specific imaging and therapy of tumor angiogenesis
Angiogenesis
Author: Strijkers, G. J., Kluza, E., et al.

Targeted liposomes, conjugated with RGD peptides, are used to visualize tumor angiogenesis by specifically binding to the avβ3 integrin on angiogenic tumor endothelium. In vivo MRI results reveal that RGD-conjugated liposomes accumulate predominantly in the tumor's rim, highlighting active angiogenesis. Conversely, nonspecific RAD-conjugated liposomes show a more uniform distribution throughout the tumor. The study emphasizes the critical role of fluorescent liposomes, as they enable the validation of MRI findings through ex vivo fluorescence microscopy. This microscopy confirms the specific association of RGD-conjugated liposomes with tumor blood vessels, while RAD-conjugated liposomes demonstrate nonspecific extravasation.

Fig. 1 In vivo visualization of tumor angiogenesis by application of avβ3 integrin-targeted paramagnetic and fluorescent liposomes Fig. 1 In vivo visualization of tumor angiogenesis by application of avβ3 integrin-targeted paramagnetic and fluorescent liposomes

FAQs

How are fluorescent liposomes used in drug delivery research?
What are the advantages of using fluorescent liposomes in research?
How can I choose the appropriate fluorescent liposome for my research?
Can I customize fluorescent liposomes to match my specific research needs?

Resources

 

For Research Use Only. Not For Clinical Use

Online Inquiry

Cat Product name Lipid Composition Data sheet MSDS Inquiry
CLBD003LY Fluorescent Liposome DiA L-alpha-Phosphatidylcholine, Cholesterol Inquiry
CLBD004LY Fluorescent Liposome DiD L-alpha-Phosphatidylcholine, Cholesterol Inquiry
CLBD005LY Fluorescent Liposome DiI L-alpha-Phosphatidylcholine, Cholesterol Inquiry
CLBD007LY Fluorescent Liposome DiR L-alpha-Phosphatidylcholine, Cholesterol Inquiry
CLBD003LYm Mannosylated Fluorescent Liposome DiA L-alpha-Phosphatidylcholine, Cholesterol Inquiry
CLBD004LYm Mannosylated Fluorescent Liposome DiD L-alpha-Phosphatidylcholine, Cholesterol Inquiry
CLBD005LYm Mannosylated Fluorescent Liposome DiI L-alpha-Phosphatidylcholine, Cholesterol Inquiry
CLBD007LYm Mannosylated Fluorescent Liposome DiR L-alpha-Phosphatidylcholine, Cholesterol Inquiry
LDLY-0123-LY209 DiD Labeled Liposomes HSPC: 56.2M
Cholesterol: 38.5M
DSPE-mPEG2000: 5.3M
Inquiry
LDLY-0123-LY210 DiR Labeled Liposomes HSPC: 56.2M
Cholesterol: 38.5M
DSPE-mPEG2000: 5.3M
Inquiry