Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.
Lipid Composition
DOTAP: 10M Chol: 75M DOPE: 5M
Applications
Cancer research; Target delivery
Format
Liquid
Hydration Solution
Deionized RNAse-free Water
External Solution
Deionized RNAse-free Water
Storage
2°C - 8°C (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Dosage
Download
DataSheet
MSDS
FAQsPublished Data Customer ReviewsRelated Sections
What unique properties does the DOTAP:Chol:DOPE (10:75:5) liposome formulation offer?
This formulation combines the cationic lipid DOTAP with cholesterol and DOPE in a specific ratio. DOTAP assists in binding and delivering genetic materials due to its positive charge, Chol provides stability to the liposomal structure, and DOPE facilitates membrane fusion, enhancing the overall delivery efficacy of the liposomes.
How are DOTAP:Chol:DOPE (10:75:5) liposomes typically used in research?
These liposomes are extensively used for the delivery of genetic materials, including DNA and RNA. Their composition makes them particularly effective for transfection in a variety of cell types, supporting research in gene therapy, molecular biology, and drug delivery systems.
Are there any specific storage or handling recommendations for these liposomes?
DOTAP:Chol:DOPE liposomes should be stored at 4°C and protected from light to maintain their integrity. They should be handled gently to prevent disruption of their structure. Freezing should be avoided as it can adversely affect liposome stability.
Can these liposomes be customized for specific research needs?
Yes, there is potential for customization in terms of encapsulating various agents or adjusting the lipid composition to tailor the liposomes for specific research applications. This flexibility allows researchers to experiment with different configurations for optimal results.
Z-average diameter and Zeta potential values of lipoplexes at different N/P molar ratios
The study delves into the utilization of DOTAP:Chol:DOPE (10:75:5) liposomes in research, emphasizing their significance in experimental settings. These liposomes are examined for their efficacy in delivering various molecules into cells, showcasing their stability and functionality through preparation and characterization techniques. Experimental data illustrates the effectiveness of DOTAP:Chol:DOPE liposomes in delivering therapeutic agents, genetic material, or imaging agents to target cells. The research underscores the importance of liposomal formulations in drug delivery systems, highlighting their potential applications in biomedical research and clinical settings. By utilizing DOTAP:Chol:DOPE liposomes, researchers aim to enhance drug bioavailability and therapeutic efficacy while minimizing side effects. This study contributes to advancing our understanding of liposomal drug delivery systems and their practical implications in biomedicine.
Lechanteur, A., Sanna, V., et al. Cationic liposomes carrying siRNA: impact of lipid composition on physicochemical properties, cytotoxicity and endosomal escape. Nanomaterials. 2018, 8(5): 270.
Optimal Cytotoxicity Balance
This liposome formulation demonstrates a keen balance in cytotoxicity, particularly at lower N/P ratios, making it suitable for delicate applications requiring cell viability preservation.
Superior Gene Therapy Applications
The unique composition of DOTAP, Chol, and DOPE, in these liposomes, renders them highly efficient for gene therapy, enhancing the cellular uptake of nucleic acids.
Advanced Transfection Capabilities
These liposomes excel in transfection efficiency, making them a preferred choice for researchers focused on nucleic acid delivery in various biological studies.
Optimal for siRNA Delivery
Highly effective for siRNA delivery, particularly at higher N/P ratios. The DOTAP:Chol:DOPE liposomes facilitate better endosomal escape and siRNA intracellular effectiveness, which is essential for our RNA interference research.
Stable and Compatible
Appreciate the stability and compatibility of these liposomes with biological membranes. This makes them highly suitable for a range of research applications, particularly in pharmacological studies.
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For Research Use Only. Not For Clinical Use