Succinyl-Liposome (PEGylated) is Liposome (PEGylated) with the carboxyl group, which can be activated by EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide) and react with sulfo-NHS (N-hydroxysulfosuccinimide) to produce a significantly more stable and more soluble active intermediate (NHS ester). The intermediate can covalently conjugate to the amine group on the proteins, peptides or antibodies through displacement of sulfo-NHS group by amine.
What chemical groups are present on Succinyl-Liposome (PEGylated)?
It contains carboxyl groups that can be activated for conjugation to amine groups on proteins, peptides, or antibodies.
Why should EDC and sulfo-NHS be prepared immediately before use?
They should be freshly prepared to ensure maximum reactivity and efficiency in forming the NHS ester intermediate.
At what pH is the reaction of sulfo-NHS-activated molecules with primary amines most efficient?
The reaction is most efficient at pH 7-8.
Why should Tris buffer be avoided during the conjugation process?
Tris buffer contains amines, which can interfere with the activation and conjugation processes by reacting with NHS esters.
What efficiency benefits does this product offer in drug delivery?
The formulation offers high stability and efficient targeting, leading to improved therapeutic outcomes.
Improved Drug Stability
The PEGylated formulation provided excellent stability to our therapeutics, improving the overall efficacy.
Superior Conjugation Performance
The carboxyl groups enabled efficient and stable conjugation with our peptides, significantly enhancing our research outcomes.
Enhanced Drug Loading
The high drug loading capacity made it easier to achieve the desired therapeutic dosages.
Improved Biodistribution
The formulation ensured better biodistribution, improving the therapeutic potential in our models.
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For Research Use Only. Not For Clinical Use 