Azide-Liposome is Liposome with the azide group, which can react with an dibenzocyclooctyne (DBCO) by click chemistry. The conjugation chemistry is based on the reaction of the azide reagent with an DBCO linker to form a stable triazole. DBCO moiety can be on the antibody and azide moiety can be on liposomes and vice versa.
Verification of antibody presence on the liposomes' surface.
This study investigates the site-specific conjugation of antibodies on the surface of liposomes. The researchers used bio-orthogonal copper-free click chemistry (SPAAC) to conjugate azide-functionalized antibodies to DBCO-functionalized liposomes. Flow cytometry results indicate that, using this approach, the antibodies were successfully and effectively conjugated to the liposome surface. Additionally, the quantity of attached antibodies was found to be proportional to the number of available DBCO groups on the liposomes. In this research, azide serves as an essential component of bio-orthogonal chemistry, enabling the selective and effective conjugation of antibodies to the liposome surface.
Gai, Meiyu, et al. "A bio-orthogonal functionalization strategy for site-specific coupling of antibodies on vesicle surfaces after self-assembly." Polymer Chemistry 11.2 (2020): 527-540.
Distributed under Open Access license CC BY 3.0, without modification.
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