Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.
Lipid Composition
DDAB: 50M Chol: 50M
Applications
Cancer research; Target delivery
Format
Liquid
Hydration Solution
Deionized RNAse-free Water
External Solution
Deionized RNAse-free Water
Storage
2°C - 8°C in dark (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
Dosage
Download
DataSheet
MSDS
FAQsPublished Data Customer ReviewsRelated Sections
What is the composition of DDAB:Chol (50:50) liposomes?
DDAB:Chol (50:50) liposomes are composed of a 1:1 ratio of dimethyldioctadecylammonium bromide (DDAB) and cholesterol (Chol). This formulation ensures stability and efficient encapsulation of various cargo molecules for research purposes.
How are DDAB:Chol (50:50) liposomes prepared?
DDAB:Chol (50:50) liposomes are typically prepared by thin-film hydration method followed by sonication or extrusion. This process ensures uniform size distribution and lipid bilayer integrity, crucial for reliable experimental outcomes.
What are the advantages of using DDAB:Chol (50:50) liposomes?
DDAB:Chol (50:50) liposomes offer several advantages, including high encapsulation efficiency, enhanced stability, and controlled release characteristics. Moreover, their cationic nature facilitates interactions with negatively charged biomolecules, aiding in targeted delivery applications.
How can DDAB:Chol (50:50) liposomes be utilized in research?
DDAB:Chol (50:50) liposomes find applications in various research areas such as drug delivery, gene therapy, and vaccine development. Researchers can use them to encapsulate and deliver therapeutic agents, nucleic acids, or imaging agents with precision and efficacy.
Are there any considerations for handling DDAB:Chol (50:50) liposomes?
Due to their cationic nature, DDAB:Chol (50:50) liposomes should be handled with caution to avoid electrostatic interactions with surfaces or biomolecules. Additionally, proper storage conditions, such as refrigeration or freeze-drying, are recommended to maintain their stability.
Comparing the transfection efficiency of different lipoplexes in HEK293 cells
The study provides an insightful exploration into the development of novel cationic liposomes composed of dimethyl dioctadecyl ammonium bromide (DDAB) and cholesterol (Chol) at a 50:50 molar ratio, aimed at enhancing the efficiency of gene delivery systems. This investigation reveals that the specific lipid composition of these liposomes significantly influences their transfection efficiency, potentially offering a more effective non-viral vector for gene therapy applications. Through meticulous experimentation, it was demonstrated that DDAB/Chol liposomes exhibit superior transfection capabilities when compared to traditional methods, with a notable reduction in cytotoxic effects. This reduction in cell toxicity, combined with high plasmid DNA entrapment efficiency, underscores the potential of these liposomes to serve as a safer and more efficient vehicle for therapeutic gene delivery. The significance of these findings lies in the potential application of DDAB/Chol liposomes in clinical settings, where safe and effective gene transfer methods are critically needed. By leveraging the unique properties of these liposomes, the study opens new avenues for the treatment of genetic disorders, providing a promising alternative to viral vectors.
Ghanbari Safari, M., & Hosseinkhani, S. Lipid composition of cationic nanoliposomes implicate on transfection efficiency. Journal of liposome research. 2013, 23(3): 174-186.
Enhanced Stability and Biocompatibility
Creative Biolabs' DDAB:Chol (50:50) liposomes offer exceptional stability and biocompatibility, crucial for our drug formulation studies. Their consistent performance surpasses other options, ensuring reliable results in our research.
Superior Encapsulation Efficiency
We've experienced unparalleled drug encapsulation efficiency with Creative Biolabs' DDAB:Chol (50:50) liposomes. Their advanced formulation enables maximum drug loading, enhancing the effectiveness of our experimental therapies.
Optimized Drug Delivery Kinetics
Creative Biolabs' DDAB:Chol (50:50) liposomes excel in delivering drugs with precise kinetics, a critical factor in our research on controlled drug release. Their reliable performance significantly advances our drug development efforts.
Seamless Integration in Research Protocols
The liposomes seamlessly integrate into our research protocols, streamlining our drug development process. Their versatility and reliability make them an indispensable tool in our studies.
Dedicated Customer Support
We appreciate the dedicated support provided by Creative Biolabs for their DDAB:Chol (50:50) liposomes. Their responsive team ensures smooth experimentation and prompt resolution of any issues, enhancing our research productivity.
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For Research Use Only. Not For Clinical Use