DOTAP Liposomes - Creative Biolabs

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What are DOTAP liposomes and their primary uses?
DOTAP liposomes are cationic liposomes, mainly utilized in transfection applications for delivering genetic materials like DNA and RNA into cells. They are particularly effective in transfecting various cell types, including primary and suspension cells, and have applications in both in vitro and in vivo settings.
What are the key considerations in preparing DOTAP liposome formulations for use in experiments?
When preparing DOTAP liposome formulations, it's important to consider the lipid-to-DNA ratio, as this can significantly affect transfection efficiency. The pH and ionic strength of the buffer used can also influence the complex formation and stability.
Can DOTAP liposomes be used in conjunction with other compounds or agents?
Yes, DOTAP liposomes can be used with other compounds. However, interactions between the liposomes and these agents must be evaluated to ensure they do not negatively affect the liposomes' integrity or the overall efficacy of the delivery system.
Are there specific cell types or conditions where DOTAP liposomes demonstrate optimal efficiency?
DOTAP liposomes are generally versatile but may exhibit varying efficiencies depending on the cell type. They are particularly effective in transfecting hard-to-transfect cells. The specific conditions, such as cell density and health, can also impact the transfection efficiency.
How can one assess the success of transfection when using DOTAP liposomes?
The success of transfection can be assessed by analyzing the expression of the delivered genetic material, using methods like quantitative PCR, Western blotting, or fluorescence microscopy, depending on the nature of the transfected material.

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ICG binds to DOTAP liposomes for enhanced optical properties

This study investigates the enhanced optical properties and tumor photoablation capabilities of Indocyanine green (ICG) when bound to DOTAP liposomes. ICG, a near-infrared dye, typically faces issues like limited water solubility and aggregation in saline solutions. By binding it to DOTAP liposomes, containing 45 mol% of the cationic lipid 12-dioleoyl-3-trimethylammonium-propane, the researchers achieved improved NIR optical properties without extra purification steps. This was evident at a dye-to-lipid mass ratio of 0.5:25, which showed complete complexation. Notably, the NIR absorption, fluorescence intensity, and photoacoustic signals of the liposome-bound dye were significantly increased. These properties were enhanced simply by mixing ICG with pre-formed DOTAP liposomes.
The practical implications of these findings are substantial. Intratumoral injection in 4T1 mammary tumors revealed that the liposome-bound ICG demonstrated superior retention compared to its free form. This enhanced retention is pivotal for effective photothermal therapy (PTT). With 808 nm laser irradiation, the study showcased effective tumor ablation with no regrowth observed for at least 30 days. The methodology presented in this study offers a straightforward yet impactful way to augment the efficacy of ICG in optical imaging and photothermal cancer treatments, potentially transforming therapeutic strategies in oncology.

Miranda, D., Wan, C., et al. Indocyanine green binds to DOTAP liposomes for enhanced optical properties and tumor photoablation. Biomaterials science. 2019, 7(8): 3158-3164.

Exceptional Delivery System for Gene Therapy
The DOTAP liposomes from Creative Biolabs have proven invaluable in our lab's gene therapy research. Their superior delivery efficiency greatly enhances gene transfection, a critical step in advancing our therapeutic strategies.
Impressive Performance in Immunization Studies
In our immunization experiments, Creative Biolabs' DOTAP liposomes demonstrated a remarkable capacity to boost antigen-specific immune responses. Their effectiveness in mucosal adjuvant activity was particularly noteworthy, enabling significant advances in our vaccine development projects.
Robust and Reliable for Biomedical Research
For anyone engaged in biomedical research, Creative Biolabs' DOTAP liposomes are a must-have. Their reliability and high performance in various applications, from drug delivery to gene therapy, have been consistently impressive in our lab's rigorous testing.
Advanced Tool for Drug Delivery Systems
In the area of targeted drug delivery, Creative Biolabs' DOTAP liposomes are able to effectively encapsulate and release therapeutic agents due to their unique composition, significantly enhancing the efficacy of our drug delivery systems.
Optimal for Nucleic Acid Delivery
We've found the DOTAP liposomes from Creative Biolabs to be exceptionally effective for nucleic acid delivery. Their ability to facilitate the transfer of DNA and RNA into target cells has been instrumental in our genetic research, outperforming other products on the market.

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DOTAP Liposomes (CAT#: LDLY-0123-LY171)