DOTAP:Chol (50:50) Liposomes - Creative Biolabs

DOTAP:Chol (50:50) Liposomes (CAT#: LDLY-0123-LY172)

Description

Cationic liposomes are used for the delivery of genetic materials such as various types of DNA and RNA. In order to capture more plasmid efficiently, the negative charge of pDNA is neutralized with positive charge of cationic lipids due to electrostatic interaction and deliver them into cells. So they are researched for use as delivery vectors in gene therapy.

Lipid Composition

DOTAP: 50M
Chol: 50M

Applications

Cancer research; Target delivery

Format

Liquid

Hydration Solution

Deionized RNAse-free Water

External Solution

Deionized RNAse-free Water

Storage

2°C - 8°C (do not freeze)

Size

100 nm

Shelf Life

6 months

Quantity

5mL (available in lyophilized powder)

Dosage

Download

DataSheet

MSDS

FAQs Published Data Customer Reviews Related Sections

How should DOTAP:Chol liposomes be stored for optimal performance?
It's crucial to store these liposomes at 4°C in the dark to maintain their stability. They should not be frozen as freezing can disrupt the liposomal structure, potentially affecting their performance.
What are the best practices for preparing DOTAP:Chol liposomes for experimental use?
When preparing these liposomes for experiments, ensure that they are thoroughly mixed and homogenized. The method of adding the therapeutic agent (e.g., nucleic acids or drugs) should be carefully chosen based on the type of agent and the desired encapsulation efficiency.
Can DOTAP:Chol liposomes be combined with other substances?
Yes, these liposomes can be used in conjunction with other substances. However, it's important to assess the compatibility of these substances with the liposomes to ensure that they do not destabilize the liposomal structure or interfere with the encapsulation and release of the therapeutic agent.
What factors should be considered when using these liposomes for transfection?
For transfection applications, consider the lipid-to-DNA ratio, the health and type of cells being transfected, and the incubation conditions. Optimizing these parameters is crucial for maximizing transfection efficiency.
How is the success of drug delivery using DOTAP:Chol liposomes evaluated?
The success can be evaluated by assessing the therapeutic response, which may include measuring the levels of delivered drug or gene expression in the target cells. Techniques such as fluorescence microscopy, PCR, and cell viability assays are commonly used.

DOTAP:Chol (50:50) Liposomes-fig1

pDNA transfection assays

This study focuses on optimizing DOTAP/cholesterol (chol) cationic lipid nanoparticles (LNPs) for delivering mRNA, plasmid DNA (pDNA), and oligonucleotides. These LNPs are a key technology in nucleic acid-based vaccines and therapies, used in the COVID-19 vaccines. The research primarily investigates the impact of DOTAP/chol molar ratios, PEGylation, and the lipid to mRNA ratio on transfection efficiency. It was found that PEGylation notably reduced mRNA transfection efficiency in DOTAP/chol LNPs. In non-PEGylated LNP formulations, a 1:3 molar ratio of DOTAP/chol exhibited the highest mRNA transfection efficiency. Moreover, an optimal ratio of 62.5 µM lipid to 1 µg mRNA was identified. Importantly, the mRNA-loaded nanoparticles remained stable and effective in transfection for 60 days at 4°C.

Additionally, the study demonstrates that DOTAP/chol LNPs can successfully transfect pDNA and oligonucleotides, indicating their potential in delivering various genetic materials into cells. The findings offer valuable insights into the formulation of DOTAP/chol cationic LNPs, paving the way for enhanced drug delivery in nucleic acid-based vaccines and therapeutic applications. This research contributes significantly to the understanding and development of lipid-based carriers for gene therapy, emphasizing the importance of optimizing lipid compositions and ratios for efficient and stable nucleic acid delivery.

Sun, M., Dang, U. J., et al. Optimization of DOTAP/chol cationic lipid nanoparticles for mRNA, pDNA, and oligonucleotide delivery. AAPS PharmSciTech. 2022, 23(5): 135.

Superior Encapsulation Efficiency
Creative Biolabs' DOTAP:Chol liposomes showcase remarkable encapsulation efficiency, ensuring optimal drug delivery in pharmaceutical research, outshining many alternatives in this vital parameter.
Enhanced Stability and Biocompatibility
The unique blend of DOTAP and Chol contributes to an enhanced stability and biocompatibility of these liposomes, making them a preferred choice for sensitive biomedical applications.
Optimal Size for Cellular Uptake
With a carefully controlled size range, these liposomes demonstrate optimal cellular uptake, crucial for effective drug and gene delivery applications.
Advanced Drug Loading Capability
The incorporation of cholesterol in the liposomes not only stabilizes the bilayer but also facilitates the loading of hydrophilic drugs, offering an advanced platform for drug delivery systems.
Ideal for Gene Delivery
Their cationic nature makes these liposomes particularly suitable for gene delivery, efficiently forming complexes with anionic nucleic acids and ensuring successful delivery into target cells.

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For Research Use Only. Not For Clinical Use

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