DOTMA Liposomes (CAT#: LDLY-0123-LY191)

DOTMA liposomes are a lipid-based delivery system used to transport DNA or RNA molecules into cells. They are positively charged due to the presence of the trimethylammonium group, which allows them to interact with negatively charged DNA or RNA molecules, facilitating their uptake by cells. These liposomes can protect DNA or RNA from degradation and immune recognition, improving their stability and half-life in the body​.

The molecular formula of DOTMA is C42H84ClNO2, and it is known for being light sensitive but not hygroscopic. It has a high purity level of over 99% and is stable for up to a year when stored at -20°C. DOTMA is soluble in ethanol, DMSO, and a mixture of chloroform:methanol at 5mg/mL​. However, DOTMA liposomes need to be stored at 4°C and cannot be frozen.

DOTMA liposomes enhance gene delivery efficiency due to their cationic nature, which facilitates efficient interactions with cell membranes. This promotes the uptake and expression of the DNA or RNA cargo inside the target cells. Their ability to form stable complexes with genetic material while protecting it from enzymatic degradation is crucial for successful gene therapy applications.

While DOTMA liposomes are effective for DNA/RNA delivery, they may induce cytotoxic effects due to their positive charge, which can activate pro-apoptotic and pro-inflammatory cell signaling pathways. Additionally, if delivered through intravenous administration, there might be a risk of opsonization, leading to their clearance from the body.

Yes, cationic liposomes like those made from DOTMA can be used for cancer drug delivery. They interact with negatively charged tumor endothelial cells, potentially inhibiting tumor growth. However, their use must be carefully evaluated due to the potential for cytotoxicity and immune responses.