Histidine as a type of Electron-rich ligand shows a relatively high affinity to bond with electropositive transition metal, Ni+2. NTA(Ni)-Liposome is Liposome with the Ni-nitrilotriacetic acid (NTA), which can be conjuagted to His-tagged proteins or peptides. The liposomes with Ni-NTA headgroups are combined with the His residues, typically at the N- or C-terminus of proteins, the proteins reversibly anchor to the liposomes.