CHOL-NSO - A Robust Mammalian Cell Expression System
Creative Biolabs is an expert in vaccine-related fields. Protein expression is an important part of vaccine development and involves numerous protein expression systems in this regard. Depending on the demand for the target protein, scientists have developed a variety of expression systems. The protein products expressed by mammalian expression systems have similar post-translational modifications and similar functions to natural eukaryotic proteins, and thus are a focus of protein expression system research.
Background
The advantage of the mammalian cell expression system is that it can guide the correct folding of the protein, provide complex N-type glycosylation and accurate type O-type glycosylation and other post-translational processing functions. Thus, its expression product is closest to natural higher biological protein molecules in terms of molecular structure, physicochemical properties, and biological functions. Therefore, the mammalian cell expression system has become a production platform for a variety of genetic engineering drugs, and it has also played an extremely important role in the discovery of new genes, protein structure and function research.
CHOL-NSO - A Robust Mammalian Cell Expression System
Normal mammalian cells are anchorage-dependent, serum-dependent, contact-inhibiting, and form-dependent. These characteristics allow them to grow in vitro for only up to 50 generations and grow in a single-layer cell mode. Higher mammalian recipient cells targeting high-efficiency expression of foreign genes should have features like loss of cell contact inhibition and anchorage-dependent characteristics, genetic stability, contain appropriate markers, fast growth and uniformity, and no synthesis and secretion of pathogenic substances, no carcinogenicity. Commonly used non-lymphoid cell lines include Chinese hamster ovary cells (CHO), small hamster kidney cells (BHK), COS cells, NSO murine myeloma line, mouse myeloma SP2/0 cells, and the like. NS0 cells are derived from the non-secreting murine myeloma and are currently used in the commercial production of therapeutic proteins. The growth of this cell line depends on the supply of cholesterol.
CHOL-NSO uses an auxotrophic NSO cell with deficiency in cholesterol synthesis resulting from lack of the HSD17B7 gene in the genome. Transfection of NSO cells with vector expressing HSD17B7 can rescue the cell's cholesterol synthesis defects, allowing cells to grow in medium without cholesterol. The advantages of CHOL-NSO are that i) stable cell lines can be produced from a chemically defined serum-free medium, ii) no cholesterol is added to the medium compared to the NS0-GS system, only soluble glutamine is added, iii) cell cycle is approximately half the cycle compared to conventional methods.
The CHOL-NSO system developed by Creative Biolabs is based on NS0 (non-secreting null) murine myeloma line, which utilizes its cholesterol synthesis defects to construct a stable cell line to make the production of eukaryotic proteins simpler and more efficient, while ensuring protein quality and reducing production cycle and cost. If you have a need for protein expression, please contact us immediately!
All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.
