Viral Vaccine Design & Optimization
- Antigen Design: Designing and optimizing vaccine antigens for specific viral pathogens to enhance immunogenicity and protective efficacy.
- Immunogenicity Prediction: Utilizing bioinformatics tools to predict antigen epitopes and T/B cell responses.
- Vector Selection: Helping clients choose the most suitable viral vector, mRNA, VLP, or recombinant protein platform.
Viral Vector Construction & Production
- Custom Vectorization: Providing custom construction services for various vectors, including adenoviruses, AAV, lentiviruses, and poxviruses.
- Viral Production and Amplification: Optimizing large-scale cell culture and viral amplification processes to ensure high-titer yields.
- Purification Services: Offering high-purity viral vaccine purification using techniques such as chromatography and ultracentrifugation.
Preclinical Immunogenicity Assessment
Molecular & Virological Assays
- Neutralizing Antibody Assays: Including Plaque Reduction Neutralization Test (PRNT) and Microneutralization Assay.
- Antibody Titer Analysis: Using methods like ELISA and ELISPOT to quantify specific antibody levels.
- Cellular Immune Assessment: Analyzing cytokine production and cytotoxic T lymphocyte (CTL) responses using Flow Cytometry and T cell proliferation assays.
- Gene Expression Analysis: Using qPCR to quantify the expression level of vaccine antigens in in vivo.
- Hemagglutination Inhibition Assay (HI): Specifically for influenza viruses and others.
