Expanded TIL Cytotoxicity Assay Service

In the development of tumor-infiltrating lymphocyte (TIL) therapy, understanding the mechanisms through which TILs can recognize and kill cancer cells is crucial. At Creative Biolabs, we are excited to offer an expanded TIL cytotoxicity assay service, designed to deliver comprehensive insights into the efficacy of TILs in targeting and destroying cancer cells.

Expanded TIL Cytotoxicity Assay Service

Our TIL cytotoxicity assay service offers advanced testing solutions to evaluate the cytotoxic activity of TILs against cancer cells. This assay is essential for researchers focused on TIL therapy, as it accurately measures the ability of TILs to recognize and eliminate tumor cells. By employing state-of-the-art technology and rigorous quality controls, our service delivers reliable and reproducible results to support both preclinical studies and clinical trials. With detailed analysis and customizable options, we provide a comprehensive approach to assess the therapeutic potential of TIL-based treatments, helping accelerate the development of effective cancer immunotherapies.

Our Advanced Cytotoxicity Assay Techniques

At Creative Biolabs, we employ a variety of advanced cytotoxicity assay techniques designed to evaluate the efficacy of TILs in recognizing and destroying tumor cells. These methods are meticulous and tailored to deliver precise and reliable data essential for advancing TIL therapies. Our cutting-edge techniques are backed by rigorous scientific research, ensuring precise and reliable results for our clients.

• Colorimetric Assays. Classic colorimetric assays, such as the MTT and XTT assays, measure cell metabolic activity as an indicator of cell viability, proliferation, and cytotoxicity. By comparing the intensity of the color, we can determine the cytotoxic potential of TILs against cancer cells.
• Chromium-51 (Cr51) Release Assay. This assay involves labeling target tumor cells with radioactive chromium-51. Despite the challenges associated with handling radioactive materials, our experienced team ensures stringent safety protocols are adhered to, guaranteeing reliable and reproducible results.
• Flow Cytometry-based Assay. This assay involves staining cells with fluorescent markers and analyzing them under a flow cytometer. It allows us to assess multiple parameters simultaneously, such as the expression of immune checkpoints, cell surface markers, and intracellular cytokines, to provide a comprehensive profile of TIL-mediated cytotoxicity.
• Fluorescence-Based Cytotoxicity Assays: These assays use various fluorescent dyes to label cellular components, facilitating the precise visualization and quantification of cell death. Fluorescence-based methods are highly sensitive and allow for multiparametric analysis, providing comprehensive data on TIL efficacy.

Fig.1 Cytotoxicity assay of expanded TILs by flow cytometry-based assay.¹

By integrating various cytotoxicity assays, Creative Biolabs provides a nuanced understanding of TIL functionality, empowering researchers to make informed decisions when advancing their therapeutic projects. If you want to learn more about our services or more cytotoxicity assays we provide, please contact us.

Tailored and Innovative Cytotoxicity Assays At Creative Biolabs

At Creative Biolabs, our team recognizes that each research project is unique, often requiring a customized approach. Our TIL cytotoxicity assay services are flexible and can be tailored to meet specific research goals. With state-of-the-art laboratory facilities and a team of highly skilled scientists, we ensure that all our assays are carried out with the utmost precision and reliability. We are committed to maintaining excellence in every aspect of our service, from project initiation to final delivery.

Reference

1. Santegoets, Saskia JAM, et al. "IL-21 promotes the expansion of CD27+ CD28+ tumor infiltrating lymphocytes with high cytotoxic potential and low collateral expansion of regulatory T cells." Journal of translational medicine 11 (2013): 1-10. Distributed under Open Access license CC BY 2.0, without modification.