High-Throughput Functional CAR-T Screening Services

Rethinking CAR-T Selection: Why Function Matters More Than Form

At Creative Biolabs, we recognize this paradigm shift. Our High-Throughput Functional CAR-T Screening Services were developed in direct response to the growing need for data-driven, scalable, and physiologically relevant platforms that enable informed decision-making early in CAR-T discovery. By integrating advanced engineering, tumor modeling, and multi-dimensional functional assays, we help researchers move beyond basic characterization to uncover CAR-T candidates with true promise.

As functional efficacy—not mere antigen recognition—defines the success of CAR-T cell therapies, high-throughput functional screening has become indispensable for early candidate evaluation and optimization. Creative Biolabs provides an end-to-end High-Throughput Functional CAR-T Screening Platform, designed to assess CAR-T cytotoxicity, cytokine secretion, exhaustion phenotype, and antigen specificity in a quantitative and scalable manner.

This platform integrates four key service components:

What Sets Our Services Apart

Translationally Relevant Functional Metrics

Unlike binding-centric assays, our platform evaluates actual effector outcomes—including killing potency, cytokine multiplex profiles (e.g., IFN-γ, IL-2, TNF-α), and exhaustion markers (PD-1, TIM-3, LAG-3).

Customizable CAR-T Engineering Framework

We utilize viral (lentiviral, retroviral) and non-viral systems to generate CAR-Ts with client-specified configurations, including:

• Costimulatory domain variation (CD28, 4-1BB, ICOS, dual signaling)
• Functional payloads (e.g., IL-15, dominant-negative TGF-βR)
• Logic-gated or switchable CARs for advanced control

This enables side-by-side comparison of multiple CAR constructs in a uniform screening context.

Diverse and Immunologically Relevant Tumor Models

Our tumor panels include:

• Luciferase/GFP-tagged lines for kinetic cytotoxicity readouts
• Antigen-density variants for avidity analysis
• PD-L1+ and TGF-β+ lines to simulate immune resistance
• 3D spheroids and matrix-embedded tumor constructs for solid tumor evaluation

These models provide insights that 2D monocultures simply cannot.

High-Throughput Screening Infrastructure

Our automated HTS workflow features:

• Robotic liquid handling and plate-based automation
• High-content imaging and IncuCyte live-cell cytotoxicity tracking
• Multiplex cytokine detection (Luminex, MSD, ELISA)
• Integrated bioinformatics for hit triage and candidate ranking

This allows reliable, scalable comparison of >100 candidates or compounds per run.

Precision Epitope Mapping with Functional Readouts

Our epitope analysis goes beyond sequence binding—it focuses on functional relevance:

• Scanning mutagenesis and peptide libraries test response specificity
• NFAT-GFP reporter CAR-Ts link epitope recognition to activation
• Cross-reactivity panels flag potential on-target/off-tumor risks early

Clients gain mechanistic confidence in their lead candidates' epitope profiles before advancing to in vivo validation.

Accelerate Your CAR-T Discovery with Functional Precision

At Creative Biolabs, we combine deep immuno-engineering expertise with scalable HTS infrastructure to de-risk and accelerate your CAR-T development. Whether you are prioritizing construct designs, evaluating drug combinations, or validating novel targets, our platform offers robust data, model flexibility, and functional depth that translate directly to better therapeutic outcomes.

Contact our CAR-T specialists today to design a custom high-throughput functional screening program for your pipeline.

Reference

1. Majumder, Avisek. "Evolving CAR-T-Cell Therapy for Cancer Treatment: From Scientific Discovery to Cures." Cancers vol. 16,1 39. Distributed under Open Access License CC BY 4.0, without modification.