VivoCAR™ Anti-CD33 (M195) h(CD28-41BB-CD3z) CAR circRNA (XS-1225-YX59)

Name: VivoCAR™ Anti-CD33 (M195) h(CD28-41BB-CD3z) CAR circRNA
SKU: XS-1225-YX59
Rating: 5 (1 reviews)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

VivoCAR™ Anti-CD33 (M195) h(CD28-41BB-CD3z) CAR circRNA leverages the M195 clone, renowned for its rapid binding and internalization properties in the bone marrow microenvironment. For in vivo applications, this clone ensures that transiently reprogrammed T cells can forcefully target AML stem cells. The circular RNA structure provides superior stability against exonucleases, facilitating a durable expression profile of the M195-CAR. This allows for precise, pulse-like administrations that maximize tumor eradication while ensuring the non-permanent nature of the therapy prevents the risk of irreversible myeloablation.

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Target Information

Target
CD33

Common Name
CD33

Alternative Names
p67, SIGLEC3, SIGLEC-3, CD33rSiglec

Full Name
Cluster of Differentiation 33

Introduction
CD33 is a transmembrane receptor expressed on myeloid progenitor cells and monocytes. Crucially, it is highly and universally expressed on blasts in over 80% of patients with Acute Myeloid Leukemia (AML). Its expression on normal hematopoietic stem cells is limited, making it a critical and validated therapeutic target for AML, despite the risk of myelotoxicity.

Targeting Diseases
Acute Myeloid Leukemia (AML), Myelodysplastic Syndromes (MDS)

UniProt ID
P20138

Gene ID
945

Target Species
Human

Antibody Clone
M195

scFv-Host Species
Mouse

Receptor_Construction
CD28-41BB-CD3z

Generation
Third

Description of Signaling Cassetes
The third-generation signaling cassette integrates two costimulatory domains, CD28 for initial robust expansion and 4-1BB for enhanced persistence, coupled with the primary CD3zeta domain for potent cytotoxic T-cell function.

Structure
Circula

Product Type
CAR IVT circRNA

Concentration
Lot specific

Storage
Aliquot and store at -80°C. Avoid freeze/thaw cycles.

Handling
Thaw and work with RNA on ice. Upon first use, pulse spin before opening and aliquot into single use portions. DO NOT vortex. Use only certified RNase-free reagents and consumables with proper RNase-free technique. Use of barrier tips is recommended. Avoid freeze/thaw cycles. DO NOT mix with media containing serum unless first complexed with a stabilizing transfection reagent.

IVT Vector Sequencing
Pass

IVT Vector Enzyme Digestion
Pass

Purity OD260/280
Pass

RNA Length and Integrity Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

RNA Sequence Assessment
Pass

Cyclization Efficiency Assessment (Determined by Denaturing Agarose Gel Electrophoresis)
Pass

Endotoxin (<10 EU/mg)
Pass

In Vitro Transfection and Expression (Optional)
Pass

Related Resources

Overview of CD33-Targeted CAR Cell Therapies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.