Reliable angiotensinogen (AGT) assays are useful for researchers studying hypertension, the renin-angiotensin system (RAS), comparing between full-length AGT and total angiotensinogen (Intact and des-Ang I), and pharmaceutical firms developing or evaluating new drugs. The customized AGT analysis services at Creative Biolabs take advantage of highly specific antibodies and perfect one-stop analysis services for global clients to support your research needs.
Background
AGT is the parent polypeptide from which angiotensin peptides are formed and its stability in urine makes it a more convenient marker of renin angiotensin system activity. AGT is synthesized primarily in cells of the proximal tubule and is secreted from the apical surface into the lumen, where it is converted first to Ang I and then Ang II by tubular renin and angiotensin-converting enzyme (ACE), respectively.
Fig.1 AGT in the pathogenesis of cardiovascular diseases.1
AGT Diagnostic Power
Normalized by urinary concentrations of creatinine, urinary angiotensinogen (UAGT) was not correlated with gender, age, race, height, body weight, fractional excretion of sodium, plasma AGT levels, or estimated glomerular filtration. UAGT has been regarded as a biomarker of RAS overactivity in kidney diseases and hypertension. Also, indirect enzymatic assay and direct radioimmunoassay or ELISA are currently used for plasma AGT measurement.
We Have Your Biomarker Assay Needs Covered
- As an experienced antibody expert, Creative Biolabs has developed a series of high-quality antibodies that can be used in the customized AGT ELISA. At Creative Biolabs, there are two AGT ELISAs that detect either total (Ang I or des-Ang I) or intact forms of AGT. Even though the cryoactivated renin in urine samples might have fragmented intact AGT into Ang I and des-Ang I AGT, the measured values of urinary AGT concentrations would not change in this AGT ELISA.
- Besides, studies also reported that LPS stimulates AGT mRNA expression and enhances AGT plasma concentrations. Creative Biolabs also provides worldwide clients with AGT mRNA analysis services.
- Moreover, G-quadruplex based biosensors can detect targets with high accuracy and speed and low cost. Employing the magnetic bead enrichment method, Creative Biolabs has constructed a reliable and efficient fluorescent biosensor platform for G-quadruplex based detection of the human AGT protein.
Our AGT analysis mainly includes but is not limited to the following:
| Cat | Service |
| BAS76-1 | AGT mRNA expression analysis |
| BAS76-2 | AGT protein detection |
| BAS76-3 | Single/Multiple-regression analyses |
| BAS76-4 | AGT polymorphisms analysis |
| BAS76-5 | AGT binding analysis |
If you are interested in our services, please don't hesitate to contact us today to develop custom solutions for specific needs. We will work to accommodate your request.
Reference
- Takeda, Yoshimichi, et al. "DNA methylation of the angiotensinogen gene, AGT, and the aldosterone synthase gene, CYP11B2 in cardiovascular diseases." International Journal of Molecular Sciences 22.9 (2021): 4587. Distributed under Open Access license CC BY 4.0, without modification.
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