Identifying and validating MACF1 as a potential biomarker for various diseases, particularly in oncology and neurodegenerative disorders.
Are you currently facing challenges in identifying novel biomarkers or validating potential drug targets? Do you struggle with the long development cycles and complexities of protein expression analysis? Our microtubule-actin crosslinking factor 1 (MACF1) analysis service helps you accelerate your drug discovery and diagnostic development processes by providing deep, actionable insights into this critical protein. Through advanced proteomics and immunoassay technologies, we offer a comprehensive solution for precise and reliable MACF1 characterization.
Introduction of MACF1 Analysis
MACF1 is a large protein scaffold that plays a fundamental role in maintaining cellular structure and function. It acts as a crucial link between the actin cytoskeleton and microtubules, governing processes such as cell-cell adhesion, cell polarity, and migration. As supported by cited literature, aberrant expression of MACF1 has been implicated in various human cancers, establishing it as a promising oncofetal biomarker. Its expression profile and various isoforms provide valuable diagnostic and prognostic information, making MACF1 a significant target in modern biomedical research.
Fig.1 Mammalian MACF1 isoforms and interactors partners.1
Workflow
01Sample Preparation & Quality Control
Upon receipt, we perform a thorough QC to assess sample integrity and suitability for downstream analysis. Samples are then processed to extract and isolate proteins, a critical step that ensures maximum yield and minimal degradation.
02MACF1 Identification & Quantification
We employ a combination of targeted mass spectrometry and highly specific antibody-based methods to accurately identify and quantify MACF1. This dual-technology approach ensures high sensitivity and specificity, allowing for reliable measurement of even low-abundance MACF1.
03Isoform and PTM Analysis
Leveraging our expertise in proteomics, we analyze different MACF1 isoforms and critical post-translational modifications (e.g., phosphorylation, acetylation) that may alter its function. This provides a deeper understanding of its biological role in disease pathways.
04Data Analysis & Bioinformatics
Our in-house bioinformatics team processes the raw data, applies rigorous statistical methods, and interprets the results in a biologically meaningful context. This step translates complex data into clear, easy-to-understand findings.
05Final Report & Consultation
We compile all findings into a detailed report, which includes raw data, analysis summaries, and biological interpretations. A dedicated specialist will then consult with you to discuss the results and their implications for your project.
Why Choose Us?
At Creative Biolabs, our commitment to scientific excellence, combined with our advanced technological platforms, sets us apart. We provide a powerful one-stop solution for all your MACF1 analysis needs. Our high-throughput capabilities ensure rapid project completion with minimal sample consumption, while our rigorous quality control measures guarantee the accuracy and reproducibility of every result. Our published data and successful collaborations with leading biopharma companies demonstrate our expertise and reliability.
Applications
Biomarker Discovery & Validation
Diagnostic & Prognostic Assay
Development: Providing the foundational data needed to develop highly specific and sensitive diagnostic and prognostic assays based on MACF1 expression levels and isoform profiles.
Drug Target Identification
Using MACF1 expression and modification data to identify and prioritize novel therapeutic targets and pathways for drug development.
Functional Research
Supporting basic biological research by providing detailed insights into how MACF1 isoforms and post-translational modifications influence cellular functions and disease mechanisms.
FAQs
-
What types of samples can you analyze for MACF1?
We have a robust workflow for analyzing a wide range of biological samples, including tissue biopsies, serum, plasma, cell lysates, and purified proteins. Our team will work with you to ensure your samples are suitable for analysis.
-
How do you ensure the specificity of your MACF1 analysis?
Our approach combines the high specificity of targeted mass spectrometry with validated antibody-based methods. This dual strategy allows us to confidently distinguish MACF1 from other proteins and accurately quantify its levels, ensuring the reliability of your results.
-
What are the main advantages of using MACF1 as a biomarker?
MACF1 is a promising biomarker due to its clear link to disease progression and its role in fundamental cellular processes. Its distinct isoforms and potential as an oncofetal marker provide unique opportunities for early diagnosis and personalized medicine that other general markers may not offer.
-
How do your MACF1 analysis services compare to standard Western blotting?
While Western blotting can provide basic protein detection, our services offer a much higher level of detail. We provide quantitative data, analyze multiple isoforms simultaneously, and can identify post-translational modifications-insights that are critical for modern biomarker and drug discovery projects.
-
Can you help with downstream validation of our findings?
Absolutely. Our services are designed to be a starting point. We can provide consultation and support for your downstream validation efforts, including custom antibody development and functional assays to confirm the biological relevance of your findings.
Related Services
Custom Antibody Development
Develop highly specific antibodies for MACF1 isoforms or other protein targets.
Quantitative Proteomics
Gain a comprehensive view of protein expression changes across your entire sample proteome.
Post-Translational Modification Analysis
A deeper dive into how modifications like phosphorylation and glycosylation affect protein function.
Our MACF1 analysis service is designed to deliver specific, high-quality data that directly informs your research and development decisions. We provide solutions for identifying MACF1 as a biomarker, quantifying its expression levels across different sample types, and validating its role in disease. These capabilities are crucial for disease diagnosis, prognosis, and the development of targeted therapies. We deliver actionable insights that help you understand the role of MACF1 in your specific biological context, eliminating guesswork and accelerating your project timeline. Creative Biolabs provides a comprehensive and reliable solution for your MACF1 analysis needs. From sample processing to final data interpretation, we deliver the insights required to propel your research forward. Our team of experts is ready to assist you.
Reference
- Cusseddu, Rebecca, Amélie Robert, and Jean-François Côté. "Strength through unity: the power of the mega-scaffold MACF1." Frontiers in Cell and Developmental Biology 9 (2021): 641727. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fcell.2021.641727
For Research Use Only.
