The APC (adenomatous polyposis coli) protein mainly play roles in regulating cell cycle and differentiation. Defective APC genes cause abnormal cell proliferation, which can lead to cancer. Creative Biolabs has launched a series of in vitro diagnostic (IVD) biomarker development and analysis services of human diseases. Here, we introduce the APC as biomarker for cancer diagnosis research.
APC Function
APC is a multifunctional protein that most prominent among its many functions is its ability to regulate β-Catenin-mediated gene transcription in response to Wnt signaling. Studies have also shown that APC plays an important role in several basic cellular processes, including regulation of epithelial cell migration and morphogenesis, as well as aspects of actin and microtubule skeleton. The loss of APC leads to dysregulation of beta-catenin resulting in an increase of β-catenin in the nucleus, which in turn activates the Wnt pathway, which is closely associated with tumor formation.
Fig.1 APC gene-associated signaling networks and interacting genes.1
APC Mutation
APC is pro-apoptotic when overexpressed in cancer cells, and anti-apoptotic when mutated. Several studies have reported germline mutations of the APC gene in patients with familial adenomatous polyposis (FAP) as well as somatic mutations in tumors developed in digestive organs (stomach, pancreas, and colon).
APC Hypermethylation in Disease
APC has been found to develop hypermethylation in a variety of diseases, especially in many types of cancers, below are the rates of methylation in several representative cancers.
60 ~ 83.6%
The incidence of APC hypermethylation in gastric cancer patients ranged from 60 to 83.6%.
11.9% ~ 62%
The methylation rate of APC promoter in colorectal cancer mucosa ranged from 11.9% to 62%.
64.91 - 84%
In approximately 64.91-84% of liver cancer patients, the APC promoter exhibits hypermethylation.
58.6%~71%
APC promoter hypermethylation was detected in 58.6% to 71% of pancreatic cancer patients.
Services Provided by Creative Biolabs
APC protein is an important cancer-related protein in Wnt signaling channel, and the expression level affects the process of cell growth, cell division and differentiation and so forth. The cell-based functional study of APC includes its complex protein and mutation site can further elucidate its pathogenic mechanism.
Our services can be customized to suit the specific needs of our clients, popular analysis services targeting APC include but are not limited to the following:
| Cat | Service |
|---|---|
| BAS113-7 | APC-KO Cell Line Construction |
| BAS113-8 | APC Cell Apoptosis Assay |
| BAS113-9 | APC Cell Proliferation Assay |
| BAS113-10 | APC Cell Cycle Assay |
| BAS113-11 | APC Cell Migration and Invasion Assay |
| Cat | Service |
|---|---|
| BAS112-6 | AP3B1 PCR Assay |
| BAS112-7 | AP3B1 Sequence Assay |
| BAS112-8 | AP3B1 RNA Isolation |
| BAS112-9 | AP3B1 Methylation Analysis |
We are committed to providing the highest quality of custom services and products at the most reasonable prices. Please feel free to contact us for more information and a formal quote.
Reference
- Zhu, Lila, et al. "APC promoter methylation in gastrointestinal cancer." Frontiers in Oncology 11 (2021): 653222. Distributed under CC BY-SA 4.0, without modification.
For Research Use Only.
