ACE2 analysis is a critical tool for identifying novel therapeutic compounds. Researchers can use our services to screen and characterize small molecules or biologics that modulate ACE2 expression or function, offering new avenues for treating viral diseases and other pathologies.
Creative Biolabs offers comprehensive ACE2-specific assays to support your drug discovery programs. Our high-throughput, customizable platforms are designed to meet your precise research needs.
Introduction of ACE2
ACE2 is a fascinating dipeptidyl carboxypeptidase, sharing significant homology with human angiotensin 1 converting enzyme. Its expression across various organs, including the cardiovascular system and kidneys, underscores its role in regulating fundamental physiological processes like blood pressure. Most importantly, ACE2 acts as a cellular receptor for the spike glycoprotein of human coronaviruses, including SARS-CoV and SARS-CoV-2. By binding to the S protein, it facilitates viral entry into host cells, making it a key focus for virology and drug development.
Fig.1 Proposed mechanism of ACE2 shedding facilitating SARS-CoV-2 ingress.1
ACE2 protein exists in two forms: a full-length, membrane-bound form and a circulating, soluble form (cACE2). The membrane-bound protein is a Type I transmembrane protein, notably absent from major immune organs like the spleen and thymus. The soluble form is produced when the metalloprotease ADAM17 sheds the full-length protein from the cell membrane. This cACE2 is a valuable biomarker, not only as an index of viral load but also for its prognostic power in various diseases. Research has linked cACE2 to the pathology of kidney and cardiovascular diseases, as well as tumors. Additionally, genetic ACE2 deficiencies are associated with heightened inflammatory responses and cardiac remodeling.
ACE2 Analysis Services at Creative Biolabs
Targeting the full-length ACE2 protein and measuring circulating blood levels provide crucial avenues for disease monitoring and the development of effective therapeutics. Plasma ACE2 activity, for instance, has been identified as a predictor of mortality in conditions like aortic stenosis, underscoring its clinical relevance. At Creative Biolabs, we offer a comprehensive suite of assays designed to support the analysis of ACE2 biomarkers, enabling you to precisely identify and quantify the effects of your drug treatments on cellular or animal models.
Our extensive analytical services include, but are not limited to:
- Cell-Based Assays
Binding and neutralization assays using cells expressing ACE2, such as HEK293/ACE2 cells, to evaluate drug interactions.
- Enzymatic Activity Assays
High-sensitivity serum ACE and ACE2 enzymatic assays to measure functional protein activity.
- Protein Detection
Comprehensive detection of serum ACE2 and other related proteins using a range of gold-standard methods, including Western Blot (WB), ELISA, Flow Cytometry (FACS), and Immunohistochemistry (IHC).
- Quantitative Fluorescence
Precise detection of ACE2 using quantitative fluorescence techniques for high-accuracy measurements.
Service Workflow
Our ACE2 analysis workflow is a multi-step process designed for seamless execution and clear communication from start to finish.
01Initial Consultation and Study Design
Our expert scientific team collaborates closely with you to define your specific research objectives and tailor a customized study plan that perfectly aligns with your project goals, ensuring every step is optimized for success.
02Sample Submission
You provide the necessary starting materials, which can include a wide range of cell-based samples, animal-derived specimens, or various other biological matrices.
03Assay Execution
Our certified technicians perform the agreed-upon ACE2 analysis assays in our state-of-the-art laboratory, following stringent, real-time quality control protocols to ensure data integrity and precision.
04Data Delivery and Reporting
We deliver a comprehensive data package and a detailed, easy-to-understand report summarizing all findings for your project in a timely manner.
05Post-Analysis Review
A follow-up consultation with your dedicated project manager is scheduled to discuss the results, their biological implications, and strategic next steps for your research, providing invaluable insights.
Applications
Drug Discovery and Development
Disease Prognosis and Monitoring
The analysis of circulating ACE2 levels provides a powerful non-invasive biomarker for disease progression. Our clients leverage this data to monitor the efficacy of treatments and predict patient outcomes in a variety of conditions, including cardiovascular disorders, chronic kidney disease, and specific cancers.
Functional Mechanism Studies
Understanding the intricate biological role of ACE2 is essential for basic research. Our assays allow for detailed investigation into the protein's function, its interactions with ligands, and its broader impact on signaling pathways and physiological processes in different model systems.
Service Highlights
- Precision and Accuracy: We utilize highly sensitive and validated assays to ensure the utmost precision in all ACE2 measurements, providing you with reliable and reproducible data that you can trust.
- Full Customization: Our expert team is able to customize any aspect of the ACE2 analysis workflow, from unique sample types to bespoke assay designs, to align perfectly with your experimental needs.
- Expert Consultation: Every project includes direct access to our team of seasoned biologists who offer guidance on study design, data interpretation, and strategic recommendations to optimize your research outcomes.
FAQs
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How do your assays differentiate between the membrane-bound and soluble forms of ACE2?
We employ a variety of specialized techniques designed to distinguish between these two critical forms of the protein. Our protocols leverage specific sample preparation methods and assay formats, such as cell-based assays that exclusively measure the membrane-bound form and ELISA-based kits that are calibrated to detect only the soluble, circulating form of ACE2 in bodily fluids, providing clarity in your data.
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Is it possible to develop a custom ACE2 assay for a novel cell line or animal model not listed in your standard protocols?
Absolutely. Our core strength is our ability to create tailor-made solutions for our clients. Our dedicated team of scientists specializes in developing and validating custom assays for unique biological systems, whether it's an uncharacterized cell line, a specific transgenic animal model, or a novel experimental setup, we are committed to providing a precise analytical solution.
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Can your service accommodate both ACE2 expression-level and functional activity analysis?
We provide a holistic view of the protein by offering assays that measure both its expression and its enzymatic activity. For expression levels, we utilize methods like ELISA and WB, while for functional analysis, we offer specialized enzymatic assays that can measure ACE2's activity in a kinetic or end-point format, giving you a complete picture of the protein's behavior.
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Is your ACE2 analysis service scalable for high-throughput screening campaigns?
Our entire platform is engineered with high-throughput compatibility in mind. Whether you need to screen a few compounds or a large library of thousands, our automated systems and optimized workflows are perfectly suited for large-scale projects, allowing you to quickly and efficiently screen for potential modulators of ACE2.
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How does your service address potential matrix effects in various biological samples?
We have extensive experience in working with complex biological matrices. To mitigate potential matrix effects, we employ specific sample dilution strategies, use carefully selected blocking buffers, and run matrix-matched controls. This meticulous approach ensures that any interference from the sample background is minimized, guaranteeing the reliability of your results.
Custom assay can be designed in 1-2 weeks at Creative Biolabs, dramatically shortening the time for assay development to weeks rather than months. If you are interested in our analysis services, please feel free to contact us for more information.
Reference
- Xiao, Li, Hiroshi Sakagami, and Nobuhiko Miwa. "ACE2: the key molecule for understanding the pathophysiology of severe and critical conditions of COVID-19: demon or angel?." Viruses 12.5 (2020): 491. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/v12050491
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