One of the causes of ATP4A-related diseases is the increase of stomach pH value caused by abnormal ATP4A, which leads to the enrichment of potential pathogenic microorganisms, or airway infection by inhalation of fine dust. Creative Biolabs provides one-stop services for further study the molecular mechanism and pathogenesis of ATP4A as a diagnostic biomarker.
H+/K+ ATPase is a heterodimeric protein, the ATP4A gene encodes the H+/K+ ATPase alpha subunit, which is one of the two subtypes. ATP4A (ATPase, H+/K+ exchanging, alpha polypeptide) protein is a kind of proton pump, completes H+/K+ transmembrane transport through its own phosphorylation and dephosphorylation, and its phosphorylation site is at Asp385.
ATP4A can bind the phosphoric acid group released by ATP decomposition, make its own conformation change, and form pores in the cell membrane that allow ion transport, thus transferring the H+ negative concentration difference bound in the cell out of the cell.
H+/K+ ATPase is mainly existing in parietal cells, which are highly specialized epithelial cells located in the inner cell wall of the stomach called the gastric mucosa. When H+/K+ ATPase is present in gastric epidermal cells, it is involved in the directed transfer of H+ out of cells to form gastric acid.
Failure of the proton pump binding in AtP4a-deficient cells prevents H+ release, resulting in changes in the concentration of protons in parietal cells. Intracellular H+ accumulation may lead to mitochondrial dysfunction and impaired oxidative phosphorylation activity, resulting in abnormal ROS signaling and inducing parietal cell atrophy.
Parietal cells have an extensive system of secretory membranes, and H+/K+ ATPase is the main protein component of these membranes. Studies have shown that abnormalities in ATP4A are associated with a variety of diseases. The follow shown some of the related diseases, mutation sites and inhibitors.
To further elucidate the mechanism of ATP4A, our highly specific ATP4A antibody is one of the effective tools for sample analysis. Our services can be customized to suit the specific needs of our clients, popular analysis services targeting ATP4A include but not limited to the following:
| Cat | Service |
|---|---|
| BAS119-1 | ATP4A Antibody Development |
| BAS119-2 | ATP4A Protein Expression |
| BAS119-3 | ATP4A PCR Assay |
| BAS119-4 | ATP4A Sequencing Assay |
| BAS119-5 | ATP4A Immunocytochemistry Assay |
| BAS119-6 | ATP4A Immunohistochemistry Assay |
| BAS119-7 | ATP4A Inhibitor Screening Assay |
| BAS119-8 | ATP4A Phosphorylation and Dephosphorylation Analysis |
| BAS119-9 | ATP4A Cell Toxicity and Viability Analysis |
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