EEF1A1 Analysis - Creative Biolabs

Creative Biolabs offers a range of cost-effective and accurate biomarker analysis services as both stand-alone and integrated service options. Custom-designed eukaryotic elongation factor 1 alpha 1 (EEF1A1) analysis services are available for global clients, along with data analysis packages.

Biomarker Information

EEF1A1 is a major member of the eukaryotic elongation factor family that regulates protein synthesis and participates in tumor progression. EEF1A1 promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. Besides, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.

It was revealed that a high level of EEF1A1 has been reported to protect against stress-induced tumor cell death, which promotes cancer cell survival. Overexpression of eEF1A1 has been described in tumors of the colon, prostate, melanomas, pancreas, lung, and breast. EEF1A1 could be a prognostic marker and a therapeutic target for chronic lymphocytic leukemia and hepatocellular carcinoma.

Fig.1 A proposed model of the signaling pathway regulating the human osteosarcoma proliferation, metastasis and apoptosis.¹

Analysis Services at Creative Biolabs

WB: Monoclonal or polyclonal antibodies could be used in your study to detect EEFA1 protein
qRT-PCR: Total RNA from provided samples and cultured cells was extracted by Trizol reagent. Reverse transcription and SYBR Green-based real-time PCR will be carried out subsequently. The primers are designed by our experienced scientists.
IHC assay
Recombinant EEF1A1 production service and function analysis

Besides the mentioned analysis services, Creative Biolabs also provides single-molecule fluorescence in-situ hybridization probes design services to detect either EEF1A1 or EEF1A2 mRNAs. Scientists at Creative Biolabs will analyze and compare the subcellular distribution of EEF1A1 and EEF1A2 mRNAs at specific developmental stages and in mature neurons, providing tailored services for global clients.