Can you differentiate between EEF1A1 and its isoform EEF1A2 in your analyses?

Yes, our specialized services include advanced techniques such as single-molecule fluorescence in-situ hybridization (FISH) probe design, which enables precise and specific detection and differentiation of both EEF1A1 and EEF1A2 mRNAs within your samples. This allows for comparative analysis of their distinct expression patterns and subcellular distributions.

What quality control measures are in place to ensure the reliability of EEF1A1 analysis results?

We implement stringent quality control measures at every stage of the EEF1A1 analysis workflow, including RNA/protein integrity checks, assay calibration, positive and negative controls for all experiments, and replicate analyses to ensure high reproducibility and the utmost reliability of all generated data.

Is your EEF1A1 analysis service quantitative, providing precise expression levels?

Absolutely, our EEF1A1 analysis services, particularly quantitative Real-Time PCR (qRT-PCR) and ELISA, are meticulously designed to provide highly quantitative measurements of EEF1A1 mRNA and protein expression levels, allowing for precise comparisons across different experimental conditions. These methods ensure accurate and reliable quantification of your biomarker.

Can your services help in identifying the subcellular localization of EEF1A1 within cells?

Yes, we offer immunohistochemistry (IHC) assays for tissue samples and immunofluorescence techniques for cell lines, which are powerful methods for precisely determining the subcellular localization of EEF1A1 protein within various cellular compartments. This provides valuable insights into its functional context and interactions.

Can EEF1A1 analysis assist in the discovery of new drug targets or prognostic markers?

Definitely, EEF1A1 analysis holds significant potential for both novel drug target discovery and the identification of new prognostic markers, especially within oncology and neurodegenerative research, as its expression and function are frequently altered in disease states. Our detailed analyses can highlight these critical changes.

How do your custom-designed EEF1A1 analysis services cater to unique research requirements?

Our custom-designed EEF1A1 analysis services are inherently flexible, allowing us to tailor specific methodologies, sample processing protocols, and data analysis approaches to meet the unique and intricate requirements of your individual research projects, ensuring highly personalized and relevant experimental outcomes.

EEF1A1 Analysis Service - Creative Biolabs

Creative Biolabs is proud to offer a comprehensive range of cost-effective and highly accurate biomarker analysis services. Our custom-designed eukaryotic elongation factor 1 alpha 1 (EEF1A1) analysis services are precisely tailored for global clients, complemented by robust data analysis packages to support your critical research needs.

Introduction of EEF1A1

EEF1A1 is a fundamental member of the eukaryotic elongation factor family, playing a pivotal role in regulating protein synthesis and influencing tumor progression. It expertly facilitates the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. As a typical housekeeping gene product, EEF1A1 is essential for maintaining normal cell proliferation and survival. Furthermore, EEF1A1 diligently protects the aminoester bond against hydrolysis, ensuring a precise match between the codon on mRNA and the anti-codon on tRNA.

Research has demonstrated that elevated EEF1A1 levels can confer protection against stress-induced tumor cell death, thereby promoting cancer cell survival. Its overexpression has been documented in various tumors, including those of the colon, prostate, melanomas, pancreas, lung, and breast, highlighting its potential as a significant prognostic marker and a therapeutic target for conditions like chronic lymphocytic leukemia and hepatocellular carcinoma.

Fig.1 A proposed model of the signaling pathway regulating the human osteosarcoma proliferation, metastasis and apoptosis.¹

EEF1A1 Analysis Services at Creative Biolabs

Besides the mentioned analysis services, Creative Biolabs also provides single-molecule fluorescence in-situ hybridization probes design services to detect either EEF1A1 or EEF1A2 mRNAs. Scientists at Creative Biolabs will analyze and compare the subcellular distribution of EEF1A1 and EEF1A2 mRNAs at specific developmental stages and in mature neurons, providing tailored services for global clients.

Service Workflow

Our EEF1A1 analysis service workflow is meticulously structured for precision and client satisfaction, guiding you through each critical step.

01Initial Consultation and Experimental Design

The process begins with a detailed initial consultation, where our expert team engages to thoroughly understand your specific research objectives and project scope. Based on this discussion, we collaboratively design a tailored experimental plan, outlining the most suitable EEF1A1 analysis methods and expected outcomes.

02Sample Submission

Following consultation, clients provide biological starting materials (e.g., fresh/frozen tissues, cell lines, RNA, protein lysates). We offer clear guidelines for sample preparation and shipment, ensuring optimal integrity upon arrival.

03Sample Processing and Assay Execution

Upon receipt, our scientists meticulously process samples using established protocols specific to your chosen analysis method. This includes precise RNA extraction (qRT-PCR), protein lysis (WB), or tissue preparation (IHC). Subsequently, selected EEF1A1 assays are rigorously executed by our skilled technicians.

04Quality Control and Data Acquisition

Throughout the analysis, stringent quality control measures are continuously implemented to ensure data integrity and reproducibility. This involves equipment calibration, appropriate controls, and duplicate analyses. High-quality data related to EEF1A1 expression, localization, or function is meticulously acquired.

05Data Analysis and Reporting

Finally, our bioinformatics specialists perform comprehensive data analysis, interpreting raw data to extract meaningful insights. A detailed, transparent report is compiled and delivered, including all raw data, processed results, and interpretations, enabling confident research advancement.

Applications

Cancer Biomarker Discovery

EEF1A1 analysis is instrumental in identifying novel cancer biomarkers, given its frequent overexpression in numerous tumor types. Understanding its precise expression profile and cellular localization can significantly aid in early disease detection, accurate classification, and improved prognosis for various malignancies.

Therapeutic Target Validation

Its crucial role in promoting cancer cell survival makes EEF1A1 a compelling therapeutic target. Our analysis services are vital for thoroughly validating potential drug candidates that modulate their activity, providing critical data for preclinical and clinical development.

Disease Mechanism Elucidation

Investigating EEF1A1's multifaceted roles, from its primary function in protein synthesis to its involvement in cytoskeletal organization and apoptosis pathways, helps elucidate complex disease mechanisms in a wide array of pathologies, offering new avenues for understanding disease progression.

Drug Development Support

Our services robustly support the entire drug development pipeline by providing essential data on how EEF1A1 levels or activity are affected by experimental compounds. This crucial information guides lead optimization and helps in assessing the efficacy and safety of novel therapeutic interventions.

Service Highlights

FAQs

If you are interested in our custom EEF1A1 analysis services, please feel free to contact us for more information.