Analyzing beta 2 microglobulin (B2M) in biological fluids has gained considerable interest in pathologies such as haematologic malignancies, renal diseases, and chronic inflammatory diseases. Armed with considerable experience in biomarker analysis, Creative Biolabs provides a diverse range of assays to analyze biomarker B2M by using an immune-enzymatic assay, chemiluminescence detection, immunoturbidimetric assay, and so forth. Our breadth of technical platforms offers singleplex or multiplex data options. Whether you decide on a highly customized assay or an off-the-shelf solution, Creative Biolabs has the immunology solution you need to further your drug development program.
Scientific Validity
B2M is a low-molecular-weight protein (11.8 KDa) which is a subunit of the major histocompatibility complex (MHC) class I. Human B2M associated with HLA Class I antigens are expressed on many types of cells including thymocytes, lymphocytes, granulocytes, monocytes, platelets, epithelial cells, and endothelial cells, but is absent on erythrocytes. Due to its small size, B2M passes through the glomerular membrane, but normally less than 1% is excreted due to reabsorption in the proximal tubules of the kidney. Its presence in biological fluids reflects a rate of cell membrane renovation and cellular turnover, and there is increasing evidence that it is a useful inflammatory biomarker in several inflammatories, hematologic, infectious, autoimmune, and neoplastic central nervous system (CNS) disorders.
Fig.1 B2M is a component of MHC class I.Distributed under CC BY 2.5, from Wiki,
without modification.
Analysis Services at Creative Biolabs
At Creative Biolabs, B2M is easily and accurately measurable using immunoturbidimetry, ELISA, and chemiluminescent immunometric assay.
Taking latex-based immunoassay, for instance, a sample is diluted and reacted with a buffer that contains latex particles coated with an antibody specific for B2M. The formation of the antibody-antigen complex during the reaction results in an increase in turbidity, the extent of which is measured as the amount of light adsorbed at 545 nm. The B2M concentration in a sample is determined by constructing a standard curve from the absorbance of a reagent blank and a single-level calibrator.
A commercial human B2M sandwich ELISA kit is also frequently used for B2M quantification. The human B2M sandwich ELISA is designed to measure the amount of the target bound between a matched antibody pair. A B2M-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, as well as controls, are then added to these wells and bound to the immobilized capture antibody. The sandwich is formed by the addition of another B2M detection antibody, and a substrate solution is added that reacts with the enzyme-antibody-B2M complex to produce a measurable signal. The intensity of this signal is directly proportional to the concentration of B2M present in the original specimen.
Fig.2 B2M analysis summary.
What Creative Biolabs Can Offer You
Whether you are envisioning a potential project or improving your current operations related to your portfolio, we can help you with innovative B2M bulk reagents. Our biomarker analysis service is furnished for academic research. If you want to order this test, please feel free to contact us for more information.
For Research Use Only.
