ABCA1 Analysis - Creative Biolabs

ATP-binding cassette subfamily A member 1 (ABCA1) is a member of ATP-binding cassette (ABC) transporters subfamily, which transport a variety of biomolecules through intra- and extracellular membranes. Creative Biolabs provides diverse ABCA1 analysis services. Our powerful analytics platforms and experienced professionals can offer various service for ABCA1 analysis and give convincing results.

Scientific Validity

ABCA1, also known as CERP, is a 220 kDa transmembrane transporter encoded by the ABCA1 gene on human chromosome 9. Being a transporter protein widely expressed in tissues, ABCA1 can export the intracellular phospholipids and cholesterol to the extracellular acceptor apolipoprotein A-I (apoA-I) to form nascent high-density lipoprotein (HDL).

As shown in Fig.1, this translocation mediated by ABCA1 is the rate- controlling step in HDL biosynthesis, which suggests that ABCA1 plays an essential role in HDL synthesis. The HDL can mediate the reverse cholesterol transport by transporting cholesterol in peripheral tissues to liver. This process can effectively clear excess cholesterol in arterial vessels to prevent the formation of atherosclerosis. Moreover, ABCA1 also regulates cholesterol and phospholipid content in the plasma, the formation of microparticle (MP) and cell signaling. As ABCA1 is involved in many biochemical processes, recent study suggested that mutations in the ABCA1 gene is closely associated with many diseases such as Tangier disease, familial HDL deficiency and atherosclerosis.

HDL and the reverse cholesterol transport. ABCA1 mediates cellular HDL efflux, and HDL surface cholesterol is esterified by LCAT. Fig.1 ABCA1 participate in HDL biosynthesis.¹

ABCA1: A biomarker of Tangier disease and other family HDL deficiency

Tangier disease is a rare genetic disorder characterized by a severe reduction in the amount of HDL in the bloodstream. The disease is caused by the mutations of ABCA1 gene, which encoded the ABCA1 transport protein. As mentioned before, ABCA1 can promote the synthesis of nascent HDL by transporting cholesterol and phospholipid to loaded on the apoA-I. However, the mutations of ABCA1 gene destroy the transport function of ABCA1, making it unable to transport these two substances efficiently, which leads to the reduction in HDL biosynthesis. Hence, ABCA1 can be an effective biomarker of Tangier disease and other family HDL deficiency.

Services at Creative Biolabs

The detection of ABCA1 protein can be mainly divided into immunological assay methods such as ELISA, LFA, CLIA, LEIA, and WB protein analysis. Meanwhile, the ABCA1 gene information can also be analyzed by protein sequencing.

Creative Biolabs provides first-class and reliable analysis service for ABCA1 identification and quantification. We have established its own research teams and R&D laboratories around the world to meet the needs of global customers. This allows us to complete the analysis and detection of different targets more quickly and professionally. In Creative Biolabs, we provide services including but not limited to the following to achieve high quality analysis of ABCA1.

ABCA1 protein level test (ELISA, LFA, CLIA, WB, LEIA, Etc.)
ABCA1 protein sequencing
ABCA1 single gene test
Other customized ABCA1 analysis services

With professional teams and full rigged technology platforms, Creative Biolabs is your best choice for academic research and product development. In recent years, with the in-depth learning of ABCA1, its relationship with diabetes, thrombosis, cancer and other complex diseases has been gradually reported. In this respect, we also provide customized services to meet your various research needs.