A2M Analysis - Creative Biolabs

Creative Biolabs has an experienced technical team and a variety of advanced technology platforms that can be used for different biomarkers analysis. Our analysis services offer a complete portfolio for your biomarker detection needs with optional strategies.

Background of A2M

Alpha-2 macroglobulin (A2M), a tetramer (720 kDa) of 4 identical subunits (180 kDa) formed by two disulfide bond-linked dimers, is a large protein found in serum and plasma, and synthesized primarily by the liver. A2M can bind to most proteases, growth factors, cytokines, and hormones. It functions as a protein inhibitor in a variety of biological functions including coagulation and fibrinolysis. In humans, levels of A2M in the blood have been associated with nephrotic syndrome and Alzheimer’s disease. In rodents, A2M modulates tumor cell adhesion, migration, and growth through inhibition of PI3K/Akt and SMAD pathways.

Fig.1 Function of A2M and hypochlorite-modified dimeric A2M.¹

Analysis Services at Creative Biolabs

A2M-Sandwich ELISA

This custom A2M sandwich ELISA assay is suitable for measuring natural and recombinant human A2M. Optimized capture and detection antibody pairs with recommended concentrations are provided to save lengthy development time.

A2M-Nephelometry

In this method, the light scattered onto the A2M antigen-antibody complexes is measured. The intensity of the measured scattered light is proportional to the amount of A2M antigen-antibody complexes in the sample under certain conditions. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength. If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume. The turbidity induced by the formation of the A2M antigen-antibody immune complex is measured at 340 nm and 700 nm.

A2M-ImmunoPCR

ImmunoPCR is a promising assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR (RT-PCR). A2M-ImmunoPCR is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of A2M in plasma, serum, and cell culture supernatants. This assay employs an antibody specific for human A2M coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and A2M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away the unbound detection affinity molecule, primers and a PCR master mix are added to the wells, and data is collected using qPCR. CT values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower CT values indicate a higher concentration of the antigen.

Service summary. Fig.2 A2M analysis summary.

Our biomarker analysis service is furnished for research use only. Not for use in diagnostic procedures. If you want to order this test, please feel free to contact us for more information.