While great strides have been made in targeted therapies, improved biomarker analysis is needed to realize the full potential of precision medicine. In the field of biomarker analysis, Creative Biolabs offers a broad spectrum of services including protein biomarker quantification, characterization, as well as a complete analysis of respective target genes or re-sequencing of individual areas of defined genes. Creative Biolabs provides comprehensive analysis services targeting ribonuclease 3 (RNASE3) with the goal to identify more biomarker signatures.
Scientific Validity
RNASE3, also known as eosinophil cationic protein (ECP), is a basic protein located in the eosinophil primary matrix. It is a highly basic and potent cytotoxic single-chain zinc-containing protein present in the granules of the eosinophilic granulocytes. RNASE3 is a single polypeptide of 133 aa with the first 40 aa necessary for membrane interfering, heparin-binding, and cytotoxic activity. Serum RNASE3 has been described as a marker reflecting activation of eosinophils in asthma, potentially correlating to airway inflammation. Collectively, it was concluded that serum RNASE3 represents an independent biomarker for coronary artery disease (CAD), acute exacerbation of chronic obstructive pulmonary disease (AECOPD), coronary slow flow (CSF), and Churg-Strauss syndrome (CSS).
Fig.1 RNASE3's specific influences on various cell types and microorganisms in vitro. (Bystrom, 2011)
Analysis Services at Creative Biolabs
- RNASE3 Binding Capacity Assay
- RNASE3- Triggered Membrane Depolarization Assay
- RNASE3 Protein Quantification
- RNASE3 Heterogeneity Analysis
- Other
- Enzymatic deglycosylation of RNASE3
- Cytotoxicity assay of purified RNASE3
RNASE3 can specifically bind with high affinity to the bacteria-wall components. The binding capacity to lipopolysaccharides (LPSs), lipid A, and peptidoglycans (PGNs) could be analyzed by a fluorescent displacement assay, and the corresponding dissociation constants could be calculated using the protein labeled with a fluorophore.
The ability of RNASE3 protein to depolarize the bacteria cytoplasmic membrane provides a direct assessment of their effect at the membrane inner level.
ELISA kits are normally used to measure the concentrations of plasma RNASE3. Creative Biolabs has developed a sensitive and specific enzyme immunoassay by using our own native RNASE3 protein and high-quality antibody materials.
Due to glycosylation of the protein, the molecular mass of RNASE3 can differ between 16 and 22 kDa. RNASE3 heterogeneity could be studied using an affinity capture assay with the surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) technique.
Our biomarker analysis service is furnished for research use only. Not for use in diagnostic procedures. If you want to order custom analysis services, please feel free to contact us for more information.
Reference
- Bystrom, J. Analyzing the eosinophil cationic protein-a clue to the function of the eosinophil granulocyte. Respiratory research. 2011, 12(1): 1-20.
For Research Use Only.
