Creative Biolabs has decades of experience providing CRO services with a skilled team of scientists who will work with you in biomarker research. Our top-of-the-line biomarker analysis service armed with state of an art technique platform includes decades of experience in in vitro diagnostics (IVD) all at competitive pricing. We now provide custom human debranching RNA Lariats 1 (DBR1) analysis services for global clients.
Scientific Validity
Human DBR1 protein consists of 544 amino acids with a conserved catalytic GNHE motif and a bipartite nuclear localization signal (NLS). DBR1 plays regulatory roles in the pre-mRNA processing events, such as pre-mRNA splicing and intron turnover. Besides, the biogenesis of non-coding RNAs in intronic regions also depends on DBR1 activity. Therefore, the cellular homeostasis and function of these non-coding RNAs may be dysregulated by DBR1 inhibition. Diseases associated with DBR1 mainly include esophageal squamous cell carcinoma (ESCC), colorectal cancer, and encephalitis.
Fig.1 Structure of human DBR1.1
Analysis Services at Creative Biolabs
- DBR1 siRNA Design
- Protein Detection
- Creation of DBR1 Point Mutant Strains
- DBR1 Single Gene Test
siRNA-mediated inhibition of expression of the human DBR1 would specifically inhibit HIV-1 replication during reverse transcription. To support the role study of DBR1 in HIV reverse transcription, Creative Biolabs provides DBR1 shRNA knockdown construct generation services for global clients. DBR1 mRNA levels will be analyzed by quantitative reverse transcription-PCR.
The detection of DBR1 protein expression in tissue chips could be performed by IHC staining. A commercial human DBR1 ELISA Kit could also be used for the in vitro quantitative measurement of human DBR1 concentrations in tissue homogenates, cell lysates, and other biological fluids.
Mutants could be created using modifications of the delitto perfetto method and the site-specific genomic (SSG) method. Analyzing mutant variants of DBR1 will be achieved by qRT-PCR.
Our single gene tests are sectioned from our high-quality NGS assay. Assays have been validated for various sample types including EDTA-blood and isolated DNA. These sample types were selected to maximize the likelihood of high-quality DNA yield.
Fig.2 The analysis approaches of DBR1.
Creative Biolabs assists you with further DBR1 analysis including but not limited to the following:
- Functional analysis of recombinant human DBR1
- Binding site characterization via ELISA
- Protein genetic engineering
- Protein expression
- Assay development
Our biomarker analysis service is furnished for academic research only. Not for use in diagnostic procedures. If you want to order this test, please feel free to contact us for more information.
Reference
- Clark, Nathaniel E., et al. "Activation of human RNA lariat debranching enzyme Dbr1 by binding protein TTDN1 occurs though an intrinsically disordered C-terminal domain." Journal of Biological Chemistry 299.9 (2023). Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
