FABP2 serves as an early and specific biomarker for intestinal damage, making it invaluable for diagnosing conditions like acute intestinal ischemia, necrotizing enterocolitis in neonates, and nonocclusive mesenteric ischemia. Its rapid release into circulation upon enterocyte injury allows for timely intervention and improved patient outcomes.
At Creative Biolabs, we are your dedicated partner in cutting-edge contract research and advanced biomarker discovery. Beyond our robust genomics and proteomics platforms, we expertly utilize metabolomics, lipidomics, glycomics, and secretomics for comprehensive biomarker analysis. We are proud to offer specialized fatty acid-binding protein 2 (FABP2) analysis services to clients worldwide, rigorously supporting your crucial research endeavors.
Introduction of FABP2
FABP2 is a low molecular weight (14-15 kDa), cytosolic, and water-soluble protein belonging to the calycin superfamily. This protein plays a vital role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 expertly mediates the absorption and transport of dietary long-chain fatty acids, showing a high affinity for saturated ones and less for unsaturated variants. It also contributes to energy homeostasis by functioning as a lipid sensor. Crucially, FABP2's swift release into systemic circulation following enterocyte injury establishes it as a valuable biomarker for diagnosing acute intestinal ischemia, including necrotizing enterocolitis and nonocclusive mesenteric ischemia.
Fig.1 FABP2 structure.Distributed under CC BY-SA 3.0, from Wiki, without modification.
FABP2 Analysis Services at Creative Biolabs
Creative Biolabs offers a comprehensive suite of analytical services designed to provide precise and actionable insights into FABP2. Our primary method for quantifying human FABP2 involves the use of sandwich ELISA products, which are expertly configured with a matched antibody pair and supplementary materials. In this robust assay, the FABP2 protein present in your samples is efficiently captured by a pre-coated antibody. Following rigorous washing steps to ensure specificity, a detection antibody tailored specifically for FABP2 is introduced. The resultant color intensity, which directly correlates with the amount of bound protein, is accurately measured at 450 nm, with a correction wavelength precisely set at 630 nm for optimal data quality.
Beyond our state-of-the-art ELISA capabilities, Creative Biolabs provides a diverse range of complementary FABP2-related services, including, but not limited to:
- Bioinformatics Analysis
In-depth analysis of FABP2 cDNA and protein sequences to uncover structural and functional insights.
- Fatty Acids Analysis
Comprehensive service for the quantification and characterization of various fatty acids in your samples.
- FABP2 mRNA Expression Analysis
Precise quantification of FABP2 gene expression levels using advanced molecular techniques.
- FACS and PAGE Analysis
Flow cytometry (FACS) and polyacrylamide gel electrophoresis (PAGE) for protein and cell-based assessments.
- Customized Nanoparticle (NP) or Quantum Dot (QD)-Based Assays
Innovative and highly sensitive assay development tailored to your specific research requirements.
Service Workflow
Our FABP2 analysis service workflow is meticulously designed to ensure clarity, efficiency, and optimal results from initial consultation to final data delivery.
01Initial Consultation & Project Design
Your service begins with a detailed discussion with our expert scientific team. We delve into your research objectives, sample types, desired outcomes, and any specific considerations. Based on this, we jointly design a tailored analytical strategy.
02Sample Submission & Preparation
Clients provide their biological samples. Upon receipt, our team performs rigorous quality control checks and prepares the samples according to optimized protocols specific to FABP2 analysis, ensuring sample integrity and readiness for assay.
03FABP2 Quantification & Analysis
Utilizing the chosen analytical method, we proceed with the precise quantification of FABP2 levels. For other services like genetic or transcriptomic analysis, samples undergo the respective specialized processing steps.
04Data Processing & Quality Control
Raw data undergoes thorough processing, normalization, and stringent quality control measures by our experienced bioinformatics specialists to ensure accuracy and reliability.
05Comprehensive Data Reporting
We compile all analytical results into a clear, comprehensive report. This report includes raw data, processed data, methodological details, quality control metrics, and relevant statistical analyses, presented in an easily interpretable format.
06Scientific Consultation & Interpretation
Post-delivery, our team remains available for further consultation. We assist you in interpreting the data, discussing its implications for your research, and planning any next steps, ensuring you gain maximum scientific value.
Applications
Intestinal Health and Injury
Metabolic Disorders and Disease Risk
Variations in the FABP2 gene, such as the common Ala54Thr polymorphism, and altered protein expression levels are increasingly linked to metabolic dysregulation. FABP2 analysis aids in understanding the predisposition to and progression of insulin resistance, type 2 diabetes, obesity, and dyslipidemia, offering avenues for personalized metabolic health strategies.
Cardiovascular Health Monitoring
Given its central role in lipid metabolism and transport, FABP2 is implicated in atherosclerosis development and cardiovascular disease risk. Monitoring FABP2 levels can contribute to a more comprehensive assessment of cardiovascular health, potentially identifying individuals at higher risk for lipid accumulation-related conditions.
Nutritional and Therapeutic Assessment
FABP2 levels can serve as a marker for evaluating the efficacy of dietary interventions, bariatric surgery, and various therapeutic compounds aimed at modulating lipid absorption or metabolic pathways. This application is crucial for assessing treatment responses and optimizing patient care or drug development.
Service Highlights
- Precision and Reliability: Our analytical methods for FABP2 are meticulously validated and rigorously controlled, guaranteeing exceptional precision and highly reproducible results that you can confidently rely on for your critical research.
- Rapid Turnaround Time: We understand the urgency of scientific discovery. Our optimized workflows and dedicated expert team ensure swift processing and rapid delivery of your FABP2 analysis results, accelerating your research timelines.
- Expert Scientific Guidance: Benefit from the profound knowledge of our experienced biologists and data scientists. We provide comprehensive consultation at every stage, from initial experimental design to nuanced data interpretation, maximizing the value of your findings.
- Tailored Analytical Solutions: Recognizing that each research project is unique, we offer highly customized FABP2 analysis solutions. Our flexible approach allows us to adapt our services precisely to your specific requirements, ensuring optimal alignment with your research goals.
FAQs
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Is it possible to analyze both FABP2 protein levels and gene expression?
Absolutely. Creative Biolabs offers comprehensive analysis, allowing you to quantify both FABP2 protein levels via ELISA or Western Blotting, and its gene expression through methods like real-time PCR or RNA sequencing, providing a multi-faceted view of FABP2 regulation. This dual approach offers deeper biological insights into the mechanisms governing FABP2 activity.
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Are there any specific sample preparation requirements to ensure accurate FABP2 analysis?
Yes, specific sample preparation requirements are crucial for accurate FABP2 analysis. For instance, proper handling to prevent sample degradation, appropriate centrifugation for plasma/serum separation, and careful storage conditions (e.g., at -80°C) are vital. Our team provides detailed guidelines upon project initiation to ensure optimal sample quality.
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Can FABP2 analysis be integrated into larger biomarker panels for complex diseases?
Certainly. FABP2 analysis can be seamlessly integrated into broader biomarker panels, particularly for complex metabolic or intestinal diseases. Its specificity for intestinal injury and involvement in lipid metabolism makes it a valuable addition to multi-marker approaches for enhanced diagnostic or prognostic accuracy.
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What quality control measures do you employ for FABP2 analysis?
We employ rigorous quality control measures, including the use of certified reagents, positive and negative controls in every assay run, inter-assay and intra-assay variation checks, and comprehensive data normalization and statistical validation to ensure the highest standards of accuracy, reliability, and reproducibility in all FABP2 analysis results.
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Are you able to develop customized FABP2 assays for unique research needs?
Yes, we specialize in developing customized FABP2 assays, including those utilizing nanoparticles or quantum dots, to address unique research requirements. Our team works closely with clients to design and validate bespoke solutions that align precisely with their specific experimental designs and analytical objectives, ensuring optimal outcomes.
If you want to order custom analysis services, please feel free to contact us for more information.
For Research Use Only.
