OS9 is a key ERAD lectin that recognizes misfolded glycoproteins, facilitating their retrotranslocation and proteasomal degradation via HRD1 ligase. In colorectal cancer, OS9 clears mutant α1-antitrypsin, preventing ER stress. Antibodies track OS9-ERAD interactions via Co-IP, revealing its role in protein quality control and disease progression.
Are you currently facing challenges in identifying and validating reliable disease biomarkers or struggling with the complexity of targeting protein-based pathways? At Creative Biolabs, our OS9 analysis services help you accelerate your research and confidently validate novel targets and biomarkers. Through our advanced proteomic analysis and high-throughput screening platforms, we provide the precise data you need to drive your projects forward and unlock new possibilities.
Overview of OS9 Analysis
OS9 is a key ER-resident lectin and a central component of the ER-associated degradation (ERAD) pathway, a crucial cellular quality control system. It recognizes and facilitates the disposal of misfolded glycoproteins. Published data suggest that OS9 is frequently overexpressed in various tumors, playing a critical role in cancer cell survival and proliferation under proteotoxic stress. This makes OS9 a highly promising biomarker and target.
Fig.1 Mammalian ERAD: the HRD1 complex.1
Streamlined Process
01Sample Preparation & Quality Control
We begin by carefully preparing your samples to ensure optimal protein integrity. Our stringent quality control measures verify sample quality before proceeding, minimizing variability and ensuring reliable results. The expected outcome is a set of high-quality samples ready for analysis.
02Protein Expression Analysis
Using advanced techniques such as ELISA and Western Blotting, we quantify the total expression levels of OS9 and its specific isoforms within your samples. This step provides a precise measurement of OS9 abundance and helps you understand its presence in various biological contexts.
03Subcellular Localization & Co-localization Studies
We employ immunofluorescence and immunohistochemistry to visualize where OS9 is located within the cell and its co-localization with other proteins. This is crucial for understanding its functional role in the ER-associated degradation (ERAD) pathway. The outcome is high-resolution imaging data with detailed spatial information.
04Functional Analysis & Inhibition Assays
For projects exploring OS9 as a target, we perform inhibition assays to study the effects of specific compounds on the OS9-mediated pathway. This step provides critical data on drug efficacy and mechanism of action.
05Comprehensive Data Analysis & Reporting
Our bioinformatics experts analyze the raw data, providing you with a detailed and easy-to-understand report. This includes statistical analysis, graphical representations, and a clear interpretation of the findings.
Applications
ERAD Pathway Insights
Hypoxia-Driven Tumorigenesis
OS9 modulates HIF-1α stability under hypoxia: it promotes hydroxylation and VHL-mediated degradation but becomes dysregulated in tumors, enabling HIF-1α-driven angiogenesis. IHC studies link high OS9 to poor survival in pancreatic cancer. Targeting OS9-HIF-1α may disrupt tumor adaptation to low oxygen.
Antiviral Immune Response
OS9 enhances innate immunity by activating IRE1α-XBP1 signaling during viral infection, boosting IFN-β production. In SARS-CoV-2 models, OS9 knockdown worsens cytokine storms. Flow cytometry confirms OS9 upregulation in infected cells, positioning it as a host restriction factor for emerging viruses.
Intestinal Barrier Integrity
OS9 preserves tight junctions via p38 MAPK signaling, preventing bacterial translocation in hypoxia-induced colitis. OS9 deficiency exacerbates IBD symptoms, while overexpression mitigates barrier dysfunction. Confocal microscopy shows OS9-TJ colocalization, supporting its therapeutic potential for gut disorders.
T-Cell Activation Control
OS9 binds Itk kinase, regulating TCR-driven calcium flux and IL-2 secretion. In autoimmune diseases, OS9 dysregulation may hyperactivate T cells. Flow cytometry reveals reduced OS9-deficient T-cell proliferation, while OS9 overexpression enhances signaling. Targeting OS9-Itk could restore immune tolerance.
Drug Resistance Biomarker
High OS9 predicts chemotherapy resistance in NSCLC by stabilizing AXL kinase, a compensatory pathway during EGFR blockade. Liquid biopsies detect OS9+ CTCs, enabling resistance monitoring. Combining OS9 inhibitors with AXL antagonists overcomes adaptive resistance in preclinical models.
Featured Services
Next-Generation Sequencing (NGS)
NGS enables high-throughput detection of OS9 gene mutations, deletions, and fusion events in tumor samples. By sequencing OS9 exons, researchers identify oncogenic variants linked to cancer progression. This approach reveals OS9's role in aberrant signaling pathways, offering insights into resistance mechanisms.
Proteomic Profiling
Mass spectrometry-based proteomics maps OS9's protein interaction network in cancer cells. Key partners include chaperones (HSP90), E3 ligases (FBXW7), and autophagy regulators (LC3). These interactions suggest OS9's involvement in protein folding homeostasis and its potential as a biomarker for proteasome inhibitor sensitivity.
FAQs
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What types of samples can be used for OS9 analysis?
We can analyze a wide range of biological samples, including patient tissue, cell lysates, and biological fluids. Our team will work with you to determine the best approach based on your specific research goals.
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How can OS9 analysis assist in my cancer research project?
Our services provide critical data on OS9 expression levels, localization, and function. This information can help you validate OS9 as a diagnostic or prognostic biomarker, screen for potential inhibitors, and understand the role of the ERAD pathway in tumor biology.
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How does your OS9 analysis compare to a standard in-house lab setup?
Our service provides a more comprehensive and reliable solution, leveraging our specialized equipment, optimized protocols, and expert team. This approach saves you significant time and resources while ensuring high-quality, reproducible results that may be difficult to achieve with a standard in-house setup.
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How do I get started with a project?
Simply reach out to our team to discuss your project specifics. We will provide a customized consultation and a detailed quote based on your requirements. This initial step is designed to ensure our services perfectly align with your scientific goals.
Contact us now by email!
Reference
- Kadowaki, Hisae, and Hideki Nishitoh. "Signaling pathways from the endoplasmic reticulum and their roles in disease." Genes 4.3 (2013): 306-333. Distributed under Open Access license CC BY 3.0, without modification. https://doi.org/10.3390/genes4030306
For Research Use Only.
