Serine/threonine-protein kinase AKT1 is a key mediator of signaling pathways involved in cell survival, proliferation, and growth. As a nimble biomarker CRO, Creative Biolabs offers customized AKT1 analysis services for global clients. We hold extensive expertise in a wide range of biomarker assay development and validation services to meet your needs. Specifically, our biomarker assay services include custom assays using proprietary or commercially available antibodies, research use only kits, and singleplex or multiplex assays.
Background
AKT subfamily is made up of three isoforms in mammals: AKT1, AKT2, and AKT3. AKT1 encodes the kinase B alpha (PKBα) protein which contains an N-terminal pleckstrin homology (PH) domain, a short α-helical linker, a kinase domain (KD), and a regulatory motif. MK-2206 is an orally available allosteric AKT1/2 inhibitor and BAY 1125976 is another AKT1/2 inhibitor that binds into an allosteric binding pocket formed by the kinase and PH domains of AKT1.
Fig.1 Schematic representation of the signaling pathways activated by AKT1(E17K).1, 3
Biomarker research to the next level?
The E319G rs12881616, E17K rs121434592, L357P rs11555432, P388T rs11555431, rs2494732, and rs3803304 single nucleotide polymorphisms (SNPs) have been identified in the AKT1 gene. Activated AKT1 phosphorylates downstream substrates regulating cell growth, metabolism, apoptosis, angiogenesis, and drug responses. Thus, AKT1 has important applications in cancer research. For instance, it is essential to know how breast cancer risk is associated with histopathological and immunohistochemical characteristics and genotype polymorphisms.
Fig.2 Location of the SNPs in the AKT1 gene.2,3
Analysis Services at Creative Biolabs
According to reported studies, the AKT1 promoter hypo-methylation and decreased gene expression were associated with breast tumors. Creative Biolabs provides the following services to support your AKT1 research.
Besides customized solutions, we also provide established human phosphorylated AKT1 ELISA assays to detect and quantify the level of AKT1. As a reliable antibody provider, our popular services including but not limited to the following:
| Cat | Service |
| BAS84-1 | AKT1 Genotyping |
| BAS84-2 | AKT1 Promoter Mutation Analysis |
| BAS84-3 | AKT1 Promoter Methylation Analysis |
| BAS84-4 | AKT1 Expression Analysis |
| BAS84-5 | AKT1-XXX Interaction Analysis |
If you are interested in our services, please don't hesitate to contact us to develop custom solutions for specific needs. We will work to accommodate your request.
References
- Chen, Ying, et al. "Effect of AKT1 (p. E17K) hotspot mutation on malignant tumorigenesis and prognosis." Frontiers in Cell and Developmental Biology 8 (2020): 573599.
- López-Cortés, Andrés, et al. "Mutational analysis of oncogenic AKT1 gene associated with breast cancer risk in the high altitude ecuadorian mestizo population." BioMed Research International 2018.1 (2018): 7463832.
- Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
