AKT2 was known as an oncogene to promote cancer development. With experienced staff and years of experience in biomarkers, metabolism, and neuroscience research, Creative Biolabs is available to aid researchers in refining their technical requirements for biomarker related studies. We are well positioned to assist you with customized assay development, validation, and solutions for sample analysis needs.
Background
AKT2, known as RAC-beta serine/threonine-protein kinase, plays an important role in the conserved phosphoinositide 3-kinase signaling pathway, downstream of the insulin receptor. Like other AKT members, AKT2 is activated in cells in response to diverse stimuli such as hormones, growth factors, and extracellular matrix components. AKT2 mediates the physiological effects of insulin in several tissues including liver, skeletal muscle, and adipose tissue.
Fig.1 Structural comparison of the three AKT isoforms.1
Biomarker Research to the Next Level?
In humans, rare penetrant mutations in the AKT2 gene have been associated with monogenic disorders of glucose metabolism, such as p.Arg274His, p.Glu17Lys, and p.P50T. Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas. Besides, AKT2 is also a useful molecular marker for predicting the prognosis of meningioma.
Analysis Services at Creative Biolabs
Studies have suggested that AKT1, AKT2, and AKT3 display isoform-specific roles in muscle and fat. With our state-of-the-art technology, Creative Biolabs is competent to support your AKT2 research with a rich inventory of diverse bioanalytical assays spanning a wide range of application areas. Our methods have been used in numerous research projects, and new analytical methods are developed continuously for our customers.
For most customized assays, anti-AKT2 monoclonal antibody (mAb) is ideal for investigators involved in neuroscience, cell signaling, cancer, and signal transduction research. Armed with rich experience in antibody development, Creative Biolabs has developed a series of anti-AKT2 mAbs which can be used to detect unphosphorylated and/or phosphorylated forms of AKT2. Various anti-AKT2 antibody conjugates are also available for assay development. The AKT cell-based ELISA is a convenient, lysate-free, high throughput, and sensitive assay kit to monitor AKT2 protein expression profile in cells.
Our AKT2 analysis services include but are not limited to the following:
| Cat | Service |
| BAS86-1 | AKT2 mRNA Analysis |
| BAS86-2 | AKT2 Protein Analysis |
| BAS86-3 | AKT2 Knockdown Assay |
| BAS86-4 | AKT2 Functional Study |
| BAS86-5 | AKT2-XXX Interaction Analysis |
If you are interested in our services, please don't hesitate to contact us to develop custom solutions for specific needs. We will work to accommodate your request.
Reference
- Martorana, Federica, et al. "AKT inhibitors: new weapons in the fight against breast cancer?" Frontiers in pharmacology 12 (2021): 662232. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.
