The peripheral anti-Müllerian hormone (AMH) level has become representative of the ovarian reserve pool and is currently a promising marker for fertility and a diagnostic marker for ovarian disorders in domestic animals. Armed with rich experience in biomarker analysis, Creative Biolabs provides custom AMH analysis services from both protein level and gene level for global clients.
Background
AMH is a dimeric glycoprotein molecule that consists of two identical subunits linked by sulfide bridges. After cleavage, the N-terminal prodomain (110-kDa) and C-terminal (25 kDa) homodimers remain associated in a noncovalent complex that bind to AMH Receptor II to activate signaling. Diagnostic tests that measure AMH levels in serum or plasma are usually quantitative sandwich-ELISA that use antibodies directed against epitopes in the stable pro-region and C-terminal mature domain.
Fig.1 effects of AMH in the gonads.1
Significance of AMH
AMH has become a hot topic for scientists due to its ability to make predictions regarding the ovarian reserve pool. Different from AFC, the strong genomic and pedigree-based heritability of AMH strengthens the case for its use as a reproductive biomarker. Serum AMH is a widely used marker of functional ovarian reserve in the assessment and treatment of infertility.
- To determine dosing of gonadotropins used for superovulation prior to in vitro fertilization.
- To determine the degree of damage to ovarian reserve by cytotoxic treatments.
- To predict proximity to menopause and potentially
Fig.2 Applications of AMH detection. (Creative Biolabs)
Biomarker Research to the Next Level?
Services
Nowadays, although reproducible automated assays with good technical performance are readily available, these assays also exhibit substantial variation and require steps to deal with complement interference. Creative Biolabs has a full suite of custom services covering from product production to diagnostic assay development. There are several essential components during the measurement of AMH, the limit of blank (LOB), the linearity, the limit of detection (LOD) and the limit of quantitation (LOQ). These values vary according to the AMH assay used.
Materials
Many biological assays use antibodies to detect AMH, such as ELISA, FACS, WB, IHC and LFIA. Creative Biolabs produces core diagnostic ingredients to support your AMH research. We validate the specificity of developed antibodies to AMH by testing them on tissues known to express AMH positively and negatively. Besides, AMH antibody pair and antigens for sandwich ELISA, LFIA, turbidimetric inhibition immunoassay (TINIA) and immunonephelometric use are available.
AMH polymorphisms is associated with the number of embryos produced. Studies demonstrated genetic variations in AMH and its receptor AMHR2 genes may influence the hormonal function in folliculogenesis, resulting in infertility. The polymorphisms G146T/Ile49Ser/rs10407022 of the AMH gene and A482G/rs200255, A10G/rs11170555, C1749G/rs2071558 and G4952A/rs3741664 of the AMHR2 gene have been reported.
Collectively, featured AMH services at Creative Biolabs include but not limited to the following:
| Cat | Service |
|---|---|
| BAS102-1 | Hormonal Assay |
| BAS102-2 | AMH Polymorphisms Analysis |
| BAS102-3 | Integrated AMH Analysis |
| BAS102-4 | AMH Antibody Development |
Advantages
Creative Biolabs offers evaluation and analysis of the potential of the proposed project based on our extensive investigation and research to support the process of decision making. Feasibility studies aim to uncover the resources required to carry through, and ultimately the prospects for effective solutions.
Our project managers are well-trained, highly motivated, and professional to bring each project from project initialization and a complete closure to be handed over to our clients.
If you are interested in our services, please don't hesitate to contact us today to develop custom solution for specific needs. We will work to accommodate your request.
Reference
- Di Clemente, Nathalie, Chrystèle Racine, and Rodolfo A. Rey. "Anti-Müllerian Hormone and Polycystic Ovary Syndrome in Women and Its Male Equivalent." Biomedicines 10.10 (2022): 2506. Distributed under CC BY-SA 4.0, without modification.
For Research Use Only.
