DNAcar Service - DNA based Nanocarrier for Vaccine Delivery

Creative Biolabs, with more than a decade of experience in vaccine research and development, has now completed a comprehensive layout from researching to commercializing vaccines. In the development of vaccine formulations, we have established a variety of vaccine delivery platforms, and customers can choose the appropriate delivery method based on the characteristics of the vaccine developed.

Background

A very important aspect of successful vaccine design is the efficient delivery of antigenic material to target tissues or target cells to induce a potent protective immune response. Rational application of the vaccine delivery vector can enhance, improve, and even alter the immune response process induced by the antigenic substance, thereby improving the efficacy of the vaccine and simplifying the vaccination procedure. Vaccine delivery vectors commonly used in research and development can be classified into biological carriers such as viruses/bacteria and chemical carriers such as liposomes. In the selection of vaccine delivery system, the effective use of the carrier's loading capacity and corresponding characteristics to maximize the immune effect of the vaccine are two issues that should be considered. Secondly, the ease of production, cost, and scope of application are also issues that should be considered when commercializing vaccines.

DNAcar- DNA-based Nanocarrier for Vaccine Delivery

As the name implies, DNAcar is a three-dimensional molecule with high branches in which monomeric subunits of DNA are linked to each other. The basic unit of DNAcar - the DNA monomer consists of two DNA strands, the central regions of which are complementary. A monomer is formed when the two strands anneal, the central portion of which is double-stranded, and the four sides are four single-chain arms. Creative Biolabs also uses chemical cross-linking to prevent chain separation. Usually, we use 7 chains to make 5 different monomers. The peripheral sequences of the two ends of the five monomers may be the same or different depending on the design. The single strand of each of the five monomers was base-paired with the other, and assembled into the first layer of DNAcar, resulting in the liberation of 12 single-stranded arms. Next, a second layer of DNAcar scaffold is formed on the basis of the first layer by hybridization and cross-linking. Typically, such bilayer DNAcar frames are 60 nm in diameter, 1000 kD, soluble in most aqueous solutions, and have been shown to be harmless to living cells. After the basic structure of DNAcar is formed, tracking labels, targeting devices, payloads (such as small molecule drugs, peptides, proteins or nucleic acids) can be inserted in the single-stranded arms or in the middle double-stranded region. Among them, the release of payload in cells is mediated by biodegradation or labile linkers of DNA. By designing the underlying DNAcar, the amount of payload to be delivered can be adjusted.

Schematic diagram of DNAcar.

Fig.1 Schematic diagram of DNAcar.

Strengths of DNAcar

  • Scalability for production
  • Stability
  • Easy to precisely control size and shape
  • Allow fine-tuning of targeting capabilities
  • Compatible with a variety of payloads

Creative Biolabs is a professional CRO that involves the development of vaccines to the entire production process. We also have a wealth of proven technology and experience in vaccine formulation development. The effective delivery of vaccine is an important issue to consider when developing vaccines. According to different needs, we have developed a variety of delivery systems, such as controlled release approach, nanoparticles, liposomes, micells, ISCOMs, etc. DNA-based DNAcar is also a new and promising platform. If you are interested in this technology, please contact us!


All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.


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All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.

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