E2Shuttle - An Endosome Escape Mediated Vaccine Delivery Approach
Creative Biolabs is a vaccine development CRO with more than a decade of history. After more than ten years of growth, we have gradually improved our vaccine research system. Now we can provide customers with professional expertise and quality service in all aspects of vaccine development, including design, research, preparation, evaluation, formulation development, and commercial production!
Background
The drug carrier is endocytosed and assembled into endosomes with cell membrane components, which are subsequently developed into mature lysosomes by a variety of mechanisms. Lysosomes have a large number of degrading enzymes and acids, which will degrade the DNA/ RNA vaccines or protein drugs in them. Therefore, an effective gene or protein carrier should promote the rapid escape of a gene or protein drug from the endosome. Most viruses enter the target cells by same endocytosis and are also encapsulated in the endosome, and then the endosome is hijacked and transferred to the replication site for replication. In order to release the genome of the virus into the cytosol of the host cell without being degraded, the virus must escape the endosome without being detected by the host. To achieve this, viruses have evolved a number of complex mechanisms. This feature of the virus provides an idea for developing a delivery method for nucleic acid vaccines.
E2Shuttle- An Endosome Escape Mediated Vaccine Delivery Approach
E2Shuttle is developed mimicking the mechanism by which viruses escape from endosomes. Virus undergoes a hydrophobic transition in the endosomes, allowing it to escape from the endosomes and release its genome into the cytoplasm. E2Shuttle uses a PEI capable of binding to nucleic acids as a backbone, and a large amount of alkyl groups and long-chain fatty acids are added to the surface to synergize. The alkyl group enables it to form a hydrophobic shell, while long chain fatty acids are able to sense the low pH of the endosomes and ultimately trigger an escape mechanism.
Features of E2Shuttle
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Works well in various types of cells
E2Shuttle efficiently delivers vaccines to various cell types including up to 120 cell types such as tumor cells, neurons, myocytes, stem cells, macrophages, epithelia and hepatocytes. - Works well for various payloads like DNA, mRNA, RNA replicons, Cas9 RNP, siRNA and antisense oligos
- Works excellently in local applications as well as systemic injection
- Safety due to which only temporary changes in cytokines and liver enzymes occur
- Intrinsic activity without the involvement of cofactors
As a professional and mature CRO for vaccine development, Creative Biolabs has also conducted extensive and in-depth explorations on vaccine delivery methods. As a result, we have successfully developed a variety of unique and efficient delivery technologies. E2Shuttle was developed based on the virus's endosome escape mechanism, and we have extensively compared and verified it, which proves that this is a way to efficiently deliver nucleic acid vaccines.
All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.
