E²Shuttle - An Endosome Escape mediated Vaccine Delivery Approach
Creative Biolabs has extensive experience in vaccine development and has helped a large number of clients successfully complete their research tasks. In the development of vaccine delivery methods, we have established a number of platforms that meet the various needs of our customers for vaccine delivery. BioFiber™ is a biodegradable, implantable vaccine delivery approach that not only ensures the biological activity of the vaccine but also supports the application to various vaccines or drugs.
Background
Generally, the use of fiber as a drug delivery carrier has the ability to deliver the drug directly to the site where treatment is required, to achieve targeted administration, and to extend the residence time of the drug at the site of administration, reducing the number of administrations, while reducing drug content of blood circulation by local administration, thus avoiding the systemic side effects, and therefore tends to be more favored than systemic administration. The process of manufacturing polymer fibers is known as spinning, a special form of extrusion that uses a spinneret to form a plurality of continuous filaments. Spinning generally has the following forms of wet spinning, dry spinning, dry jet-wet spinning, melt spinning, gel spinning, and electrospinning.
BioFiber™ - Biodegradable, Implantable, Removable and Drug Release Sustained
BioFiber™ is a proprietary technology from Creative Biolabs that produces fiber at room temperature, thus ensuring that the sensitive drug it carries can maintain its activity, and because the material is biodegradable, its safety is guaranteed. BioFiber™ is arguably a universal drug delivery method. In the first place, this technology can be applied to the fibrosis of most polymers on the market (for example, PLLA, PLGA, PDO, PDLL, PCL, etc.) and prepared into a useful drug delivery carrier; second, this technology can be applied to a variety of drug types including vaccines, as well as antibiotics, growth factors, oligonucleotides, anti-tumor drugs, proteins, enzymes, and even intact viruses.
Highlight Strengths of BioFiber™
- Extended therapeutic window
- Unparalleled targeting and site stability
- Controllable, uniform release of the vaccine
- Enables simultaneous delivery of multiple vaccines in a single fiber
- Removable when needed in case of adverse reaction in the rare case
The fibrotic drug carrier produced by BioFiber™ technology is a long cylindrical structure that provides a slower rate of drug release compared to microspheres and nanoparticles that have spherical structures of the same radius, thereby making the therapeutic window for similar drug concentrations inherently longer.
The vaccine formulation prepared by BioFiber™ can be administered in an implantable manner and can be immobilized at the site of administration and maintained stable, which ensures that the vaccine is targeted to a particular tissue.
Adequate validation experiments have demonstrated that the BioFiber™ form of administration is a controlled process compared to other forms and that it ensures the uniform drug distribution.
By preparing multiple-layered, coaxial fibers, and loading different drugs in each layer, one fiber can deliver a variety of different vaccines.
In addition to a large number of advanced technologies in vaccine research, Creative Biolabs is committed to developing a variety of delivery methods for vaccines that can be used for different purposes. BioFiber™ is a unique technology that enables fibrosis of the polymer in a gentle manner and loads the drug thereon while enabling implantable, targeted, topical, sustained release administration.
Creative Biolabs is a vaccine development CRO with more than a decade of history. After more than ten years of growth, we have gradually improved our vaccine research system. Now we can provide customers with professional expertise and quality service in all aspects of vaccine development, including design, research, preparation, evaluation, formulation development, and commercial production!
Background
The drug carrier is endocytosed and assembled into endosomes with cell membrane components, which are subsequently developed into mature lysosomes by a variety of mechanisms. Lysosomes have a large number of degrading enzymes and acids, which will degrade the DNA/ RNA vaccines or protein drugs in them. Therefore, an effective gene or protein carrier should promote the rapid escape of a gene or protein drug from the endosome. Most viruses enter the target cells by same endocytosis and are also encapsulated in the endosome, and then the endosome is hijacked and transferred to the replication site for replication. In order to release the genome of the virus into the cytosol of the host cell without being degraded, the virus must escape the endosome without being detected by the host. To achieve this, viruses have evolved a number of complex mechanisms. This feature of the virus provides an idea for developing a delivery method for nucleic acid vaccines.
E2Shuttle- An Endosome Escape Mediated Vaccine Delivery Approach
E2Shuttle is developed mimicking the mechanism by which viruses escape from endosomes. Virus undergoes a hydrophobic transition in the endosomes, allowing it to escape from the endosomes and release its genome into the cytoplasm. E2Shuttle uses a PEI capable of binding to nucleic acids as a backbone, and a large amount of alkyl groups and long-chain fatty acids are added to the surface to synergize. The alkyl group enables it to form a hydrophobic shell, while long chain fatty acids are able to sense the low pH of the endosomes and ultimately trigger an escape mechanism.
Features of E2Shuttle
-
Works well in various types of cells
E2Shuttle efficiently delivers vaccines to various cell types including up to 120 cell types such as tumor cells, neurons, myocytes, stem cells, macrophages, epithelia and hepatocytes. - Works well for various payloads like DNA, mRNA, RNA replicons, Cas9 RNP, siRNA and antisense oligos
- Works excellently in local applications as well as systemic injection
- Safety due to which only temporary changes in cytokines and liver enzymes occur
- Intrinsic activity without the involvement of cofactors
As a professional and mature CRO for vaccine development, Creative Biolabs has also conducted extensive and in-depth explorations on vaccine delivery methods. As a result, we have successfully developed a variety of unique and efficient delivery technologies. E2Shuttle was developed based on the virus's endosome escape mechanism, and we have extensively compared and verified it, which proves that this is a way to efficiently deliver nucleic acid vaccines.
All of our products can only be used for research purposes. These vaccine ingredients CANNOT be used directly on humans or animals.
