Anti-CD99 (MIC2/1495R) h(41BB-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
CD99

Targeting Cell Type
T cell

Targeting Diseases
Glioma

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-41BB-CD3ζ

Discription of Signaling Cassetes
41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

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Published Data

Complete CAR data FCM

Fig.1 Expansion of CAR-T cell.

CAR Construction : 12E7-CD28-41BB-CD3ζ

Fig.1 Expansion of CAR-T cell.

Expansion fold change of CAR positive cells for 12 days.

Shi, J., Zhang, Z., Cen, H., Wu, H., Zhang, S., Liu, J., ... & Zhu, H. (2021). CAR T cells targeting CD99 as an approach to eradicate T-cell acute lymphoblastic leukemia without normal blood cells toxicity. Journal of hematology & oncology, 14(1), 1-5.

Complete CAR data Cyt

Fig.2 Anti-CD99 CAR T cells showed specifically target the T-ALL cell lines and primary cells but with minimal killing of normal blood cells.

CAR Construction : 12E7-CD28-41BB-CD3ζ

Fig.2 Anti-CD99 CAR T cells showed specifically target the T-ALL cell lines and primary cells but with minimal killing of normal blood cells.

In vitro cytotoxic activity of anti-CD99 CAR T cells against different normal blood cells (top picture), T-ALL cell lines (Jurkat/ CUTLL-1) (medium picture) and T-ALL patients' blasts (bottom picture).

Complete CAR data BI

Fig.3 Efcacy and specifcity of anti-CD99 CAR T cells in cell line-derived xenograft models of T-ALL.

CAR Construction : 12E7-CD28-41BB-CD3ζ

Fig.3 Efcacy and specifcity of anti-CD99 CAR T cells in cell line-derived xenograft models of T-ALL.

Tumor progression was monitored using bioluminescent imaging.

Complete CAR data FuncS

Fig.4 Kaplan-Meier survival curves.

CAR Construction : 12E7-CD28-41BB-CD3ζ

Fig.4 Kaplan-Meier survival curves.

Kaplan-Meier survival curves of Jurkat cell line-derived xenograft mice, MOLT-4 cell line-derived xenograft mice,nonETP ALL patient-derived xenograft mice and ETP ALL mice treated with T cells or anti-CD99 CAR T cells.

CAR scFv data BLI

Fig.5 Binding kinetics of anti-CD99 scFv with CD99 protein.

CAR Construction :

Fig.5 Binding kinetics of anti-CD99 scFv with CD99 protein.

Analysis of the interaction between the 12E7 scFv and CD99 protein using BLItz biolayer interferometry

More Published Data More Published Data

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-CD99 (MIC2/1495R) h(41BB-CD3ζ) CAR, pCDCAR1 (CAR-CQ0323)

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