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Anti-VSV Peptide T cell receptor ((N30.7 TCRα)-3), pCDTCR1 (TCR-YC1363)

It was created a transgenic mouse that expressed exclusively the TCR α-chain of a VSV-specific, H-2Kb-restricted T cell clone (N30.7, Vα2/Vβ13). When the transgenic mice were immunized with the VSV peptide, Vβ13 was again the predominant Vβ element present in VSV-specific CTL populations. However, the TCR Vβ usage was profoundly altered when these transgenic mice were immunized with peptides carrying single replacements near the C terminus of VSV. Peptides with replacements at the N terminus of VSV were able to induce a strong cytotoxic response but did not alter the TCR Vβ usage.

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Details

  • Target
  • VSV Peptide
  • Epitope
  • RGYVYDGL
  • Format
  • Non-Modified TCR
  • HLA
  • H2-Kb
  • Vector Name
  • pCDTCR1
  • Vector Length
  • ~ 8 kb
  • Vector Type
  • Lentiviral vector
  • TCR Clone
  • N30.7 TCRα
  • TCR Host Animal
  • Mouse

Target

  • Introduction
  • The VSV peptide, corresponding to residues 52–59 from the nucleocapsid of the vesicular stomatitis virus, binds to H-2Kb, triggering a strong immune response mediated by cytotoxic CD8+ T cells. Previous analysis of VSV-specific CTL clones revealed the preferential usage of the Vβ13 and Vβ8 elements. Functional studies and analysis of the crystal structure of the H-2Kb/VSV complex have allowed the potential TCR-contact residues of the VSV peptide to be identified. In the present work, we asked how single amino acid replacements in TCR-exposed positions of the VSV peptide would affect the TCR repertoire of the responding CD8+ T cell population.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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