Antibody Specificity Profile Assay

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Antibody-based therapeutics are of great value for the treatment of human diseases. In addition to functional activity, affinity, or physico-chemical properties, antibody specificity is considered to be one of the most crucial attributes for safety and efficacy. Creative Biolabs provides antibody specificity profile assay to determine the antibody reactivity against human leukocyte antigen (HLA).

Antibody Microarray

Antibody microarray, also known as antibody array, is a specific form of protein microarray. In this technology, a collection of captured antibodies is spotted and fixed on a solid surface including glass, plastic, membrane, or silicon chip, and the interaction between the antibody and its target antigen is detected. Antibody microarray is often used for detecting protein expression from various biofluids including serum, plasma, and cell or tissue lysates.

Fig.1 Sample of antibody microarray creations and
detections. (Wikipedia)

Antibody Specificity Profiling

Antibody specificity profiling is used to examine antibody reactivity against lots of human protein targets under either native or denaturing conditions. Since these putative targets are relatively homogeneous with regard to concentration and represent the vast majority of the human proteome, antibody specificity profiling represents the most unbiased and informative means to evaluate antibody sensitivity and specificity. Antibody specificity profiling is often used for verifying reagents critical to basic science or prioritizing therapeutic large molecules.

Our Service

Characterization of protein interactions on human protein microarrays in an in vitro antibody specificity profiling assay.
Probing of microarrays at two antibody concentrations, in singlicate, using a total of two microarrays, and detection of interactions using appropriate detection reagent.
Probing of one microarray with detection reagent only as a negative control.
Probing of one microarray using anti-CAMK2A and detection reagent as a positive control.

Tips: When multiple antibodies are profiled simultaneously, only one positive and negative control will be performed.

Timeline: 4 to 6 weeks

Delivery:

A comprehensive project report that includes all data of interacting proteins and results of the positive and negative controls for the antibody specificity profiling assay.
Image files and raw data.

As a leader in HLA antibody detection service, Creative Biolabs offers a full range portfolio of assays for low- to high-resolution detection. We also offer the most cost-effective multiplex assays and a wide range of singleplex kits. For more information, please feel free to contact us directly.

Reference

Wilson, J.J.; et al. Chapter seven-antibody arrays in biomarker discovery. Advances in Clinical Chemistry. 2015, 69: 255–324.

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