As an excellent service leader in the field of antibody development and immunotherapy, Creative Biolabs now offers one-stop CAR-T therapy development service which has become a promising approach to antitumor therapy. Antigen specific T cell activation assay is one of the technologies we can provide during the one-stop service which can activate CAR-T cells for further downstream research or clinical trials.
T cell activation requires at least two signals to be fully activated. The first signal is provided by the interaction of T cell antigen-specific receptor and the antigen-major histocompatibility complex (MHC). The second signal is provided by co-stimulatory receptors like CD28. In engineered CAR-T cell, CAR consists of specific scFv and signaling endodomains of CD3ζ, CD28, and 4-1BB. Creative Biolabs offers one-stop CAR-T service from basic research to clinical trials and beyond, and antigen specific T cell activation assay has been a vital technology in evaluating the activation activities of different CAR-T cell products at preclinical stage.
Assay of Antigen Specific T Cell Activation
CAR-modified T cells can recognize tumor cells via binding of the CAR to its tumor-associated antigen (TAA) independent of TCR-MHC/peptide interactions, and the engineered signaling endodomains can help T cells be activated and persistent. As a result, T cells are activated and thus they can efficiently eliminate tumor cells by the secretion of perforin and granzymes as well as the expression of FasL and tumor necrosis factor-related apoptosis inducing ligand (TRIAL). In addition, other tumor-infiltrating immune cells can be activated by the secretion of various cytokines (see Fig. 1). Creative Biolabs offers this assay to detect the following downstream activities to reflect the tumor antigen specific T cell activation.
➢ T Cell Expansion
➢ Upregulation of T Cell Surface Activation Markers
➢ Differentiation Into Effector Cells
➢ Cytokine Production or Cytotoxicity
➢ Induction of Apoptosis
Figure 1 Antitumor effects mediated by activated CAR-T cells. (Cartellieri, et.al. 2010)
Highlight Features of Our Service
➢ Senior Ph.D. scientists and extensive experience
➢ One-stop service
➢ Superior quality
➢ Timely troubleshooting
➢ Customized assay design
With years of extensive experience in the field of immunotherapy, Creative Biolabs brings our clients a suite of relevant immune-oncology assays for a wide range of projects. We aim to work closely with our clients to tailor each assay to facilitate the projects and help answer their interesting scientific questions. Creative Biolabs offers not only highly advanced assays but also customized assays. Please don’t hesitate to let us know your requirement, and we will be pleased to serve you wholeheartedly.
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