Artificial T Cell Stimulator Function Characterization Service

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The Emergency of Artificial Antigen-presenting Cells

The potential of T cells to recognize and eradicate cancer has fueled research into developing robust methods for generating large quantities of tumor-specific T cells for cancer immunotherapy. Recognizing that the signals received by T cells from antigen-presenting cells during tumor antigen exposure significantly impact their function and therapeutic effectiveness, researchers are actively developing artificial T cell stimulators. These engineered platforms aim to precisely control the signals delivered to T cells, enabling the generation of highly effective and tailored T cell therapies for cancer treatment.

Artificial T Cell Stimulator Classification

Based on the origin of artificial T cell stimulators, they can be broadly classified into:

Acellular core artificial T cell stimulator: These are entirely synthetic constructs, often composed of nanoparticles, polymers, or other biocompatible materials.
Cellular core artificial T cell stimulator: These are derived from various cell sources.

Fig.1 Different strategies for active cancer immunotherapy. Fig.1 Various active cancer immunotherapy approaches.^1,3

Artificial T Cell Stimulator Function Characterization Service at Creative Biolabs

Creative Biolabs offers a comprehensive artificial T cell stimulator function characterization service to evaluate the safety and efficacy of artificial T cell stimulators. Our services cover the interaction between artificial T cell stimulators and T cells, antigen presentation ability, co-stimulatory molecule expression, cytokine secretion spectrum, and other aspects. Through a series of rigorous in vitro and in vivo experiments, we can accurately quantify the extent to which artificial T cell stimulator induces T cell activation, proliferation, differentiation, and effector function, as well as evaluate its anti-tumor activity in tumor models. In addition, we also provide customized services to meet the different needs of customers in the artificial T cell stimulator development process, aiding customers in accelerating the development of new-generation immunotherapy products.

Artificial T cell stimulator phenotype analysis:	We implement comprehensive analysis of artificial T cell stimulator surface markers, co-stimulatory molecules, and cytokine expression by flow cytometry, immunofluorescence, etc.
Antigen presentation ability assessment:	We characterize the ability of artificial T cell stimulators to induce T cell activation, proliferation, and differentiation via ELISPOT, flow cytometry, and other methods.
T cell effector function detection:	Through cytotoxicity experiments, cytokine detection, and other methods, we can assess the killing activity of T cells, cytokine secretion levels, etc.
In vivo anti-tumor activity evaluation:	We employ animal models to assess the anti-tumor effect of artificial T cell stimulator-induced T cells in vivo.

Our Advantages

A research team with a solid artificial T cell stimulator academic foundation and research experience.
Comprehensive characterization services ranging from in vitro phenotype identification to in vivo functional characterization.
Customized solutions and project implementation according to different needs.
Professional team collaboration, an efficient experimental method, and innovative instruments and equipment provide fast results delivery.

Representative Data

Background: NK cells, despite their potent anti-tumor activity, face hurdles in clinical translation. CAR-NK cells show promise, but limitations include suboptimal in vitro expansion, low frequencies in peripheral blood/cord blood, and challenges in achieving consistent and robust therapeutic responses.

Summary: This study engineered HLA-deficient K562 cells to express CD48, 4-1BBL, and mbIL21, creating a universal T cell stimulator that potently stimulates NK cell expansion. The results reveal that co-culture with universal T cell stimulators significantly expanded both non-transduced and CAR-transduced cord blood-derived NK cells with high purity and without signs of exhaustion. Importantly, universal T cell stimulator-expanded NK cells exhibited enhanced metabolic fitness and superior antitumor activity compared to conventionally cultured counterparts.

Phenotypic Characterization

Fig.2 Phenotypic characterization of universal T cell stimulator. Fig.2 Universal T cell stimulator phenotyping.^2,4

Function Characterization

Fig.3 Universal T cell stimulator promotes the proliferation and cytotoxicity of research-grade NT and iC9/CAR19/IL-15 NK cells. Fig.3 The expansion and cytotoxicity of research-grade NT and iC9/CAR19/IL-15 NK cells are stimulated by universal T cell stimulator.^2,4

If you are interested in our comprehensive artificial T cell stimulator function characterization service, please feel free to get in touch with us.

References

Eggermont, Loek J., et al. "Towards efficient cancer immunotherapy: advances in developing artificial antigen-presenting cells." Trends in Biotechnology 32.9 (2014): 456-465.
Liu, Enli, et al. "GMP-compliant universal antigen presenting cells (uAPC) promote the metabolic fitness and antitumor activity of armored cord blood CAR-NK cells." Frontiers in Immunology 12 (2021): 626098.
Distributed under Open Access License CC BY 3.0, without modification.
Distributed under Open Access License CC BY 4.0, without modification.

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.