Bead Engulfment Assay based Phagocytosis Evaluation Service

Are you currently facing challenges related to high data variability, complex target preparation, or low throughput in evaluating antibody-dependent cellular phagocytosis (ADCP) or the function of macrophages? Creative Biolabs' Bead Engulfment Assay based Phagocytosis Evaluation Service helps you obtain highly reproducible, quantitative functional data through an advanced, flow cytometry-based microparticle platform, significantly reducing the complexity associated with native cell- or pathogen-based assays. The standardized results we delivered are crucial for therapeutic and vaccine development.

Introduction

Phagocytosis, the process by which immune cells engulf and clear cellular debris, pathogens, and opsonized targets, is a critical component of the innate and adaptive immune response. Traditional methods often suffer from high variability and low throughput, especially when using complex or difficult-to-culture biological targets. The bead engulfment assay (BEA) overcomes these limitations by utilizing standardized, functionalized fluorescent microparticles as stable, uniform surrogates. This flow-cytometry-based approach enables rapid, quantitative, and highly reproducible measurement of ADCP efficacy in therapeutic and vaccine development.

Fig.1 Strategies for macrophage-mediated tumor cell phagocytosis.¹

Bead Engulfment Assay based Phagocytosis Evaluation Service at Creative Biolabs

Creative Biolabs' Bead Engulfment Assay based Phagocytosis Evaluation Service is designed to precisely measure the functional capacity of therapeutic antibodies or plasma samples to induce phagocytosis. By employing standardized, functionalized fluorescent beads as surrogate targets, we eliminate the high biological variability inherent in using native infected cells or complex primary cell lines. We provide quantitative, reproducible data essential for advancing drug candidates and validating vaccine efficacy in high-throughput formats.

What we can offer

We provide precise phagocyte functional analysis by employing standardized, quantifiable beads that simulate physiologically relevant targets, which enables unbiased quantification of phagocytic activity in key immune cells. Our core technical strengths lie in:

In addition to our diverse range of phagocytosis assays, which utilize targets such as bacteria, beads, and tumor cells, we also provide specialized CAR-MA-mediated Trogocytosis Assay and Eating Assay services to support your research.

Our Service Process

Required starting materials:

• Candidate therapeutic antibodies (purified IgG, Fab fragments, or complex fusion proteins).
• Target antigens (purified recombinant protein, or specific peptide/ligand sequence).
• Phagocytic cell line of interest (e.g., THP-1 or specified primary cell source).

Key Steps Involved:

Final Deliverables:

• Comprehensive Study Report: Detailed protocol, reagent specifications, flow cytometry gating strategy, and raw data files (FCS files).
• Phagocytosis Efficacy Data: Calculated Percent Phagocytosis and the statistically analyzed Phagocytic Index (quantitation of beads internalized per cell).

Key Advantages

• Superior Reproducibility: Our use of standardized, pre-functionalized microparticles bypasses the high biological variability associated with native targets, ensuring low CVs and high-fidelity results.
• Flow Cytometry Quantitation: We go beyond a simple percentage, using advanced flow analysis to provide a quantitative Phagocytic Index—the true measure of efficacy that relates antibody concentration to bead number internalized per cell.
• Broad Versatility and Application: The assay is proven effective across diverse fields, including Oncology, Vaccine Development, and Neuroimmunology.

FAQ

Why Choose Us?

Creative Biolabs accelerates your therapeutic development with our reliable, quantitative Bead Engulfment Assay. Our standardized, flow cytometry-optimized platform minimizes experimental variability, delivering the robust, actionable data you need to streamline pipeline decisions.

Customer Reviews

"Using Creative Biolabs' Bead Engulfment Assay based Phagocytosis Evaluation Service in our research has significantly improved the reproducibility of our ADCP data for new IgG isotypes, allowing us to confidently move our lead molecules forward."— Dr. N***n R***e.

"The ability to reliably quantify phagocytosis in primary microglial cultures using this service was a game-changer. We finally have a high-throughput method that gives us clear, quantitative data on CNS immune cell function, which was previously a major bottleneck."— E***y M***s.

"We needed to screen hundreds of patient plasma samples for opsonic activity against a recombinant pathogen antigen. Creative Biolabs delivered a highly repeatable, flow-optimized solution that allowed us to efficiently process the entire cohort in a fraction of the time compared to using traditional methods."— Jl Fr.

How to contact us?

Ready to standardize your phagocytosis assessment? Reach out to our specialists today to review your project needs, obtain a comprehensive quotation, and explore how our dedicated bead assay services can enhance your upcoming research.

Contact Our Team to Learn More and Advance Your Project.

Reference

1. Chen, Siqi et al. "Harnessing and Enhancing Macrophage Phagocytosis for Cancer Therapy." Frontiers in immunology vol. 12 635173. Distributed under Open Access License CC BY 4.0, without modification. https://doi.org/10.3389/fimmu.2021.635173.