Based on the outstanding expertise and extensive experience, Creative Biolabs has specially developed a systematic approach of multiplex cytokine and chemokine assays for research and clinical purposes. Our multiplex cytokine and chemokine assay services are designed to help you detect cytokines/chemokines of cells that can produce cytokine/chemokine, including immune cells, known as macrophages, B cells, and T cells, as well as endothelial cells, fibroblasts, and various stromal cells.
Introduction of Cytokine and Chemokine
Cytokines are a wide class of small proteins that play a role in various cell signaling especially in paracrine signaling, autocrine signaling, and endocrine signaling as immunomodulating agents. Their release affects the behavior of cells around them. Cytokines may include chemokines, interleukins, lymphokines, interferons, and tumor necrosis factors, but not include hormones or growth factors. A given cytokine can be released by more than one type of cell. Generally, the megascopic function of cytokines is to maintain and regulate the balance between humoral and cell-based immune responses. Chemokines are a type of cytokines able to induce directed chemotaxis in nearby responsive cells.
Multiplex Cytokine and Chemokine Assay
Multiplex technologies make it available to detect multiple biomarkers simultaneously. Multiplex cytokine and chemokine assay is a useful tool to analyze coordinate immune responses. Additionally, it is also powerful for application to vaccine development and monitoring of efficacy. In practice, cytokines and chemokines are usually detected in vitro using the preclinical human cells to predict the ability for a new drug to induce considerable cytokine release from immune cells in vivo. Fully understood these needs, Creative Biolabs has established two different platforms to conduct multiplex cytokine and chemokine assays, both of which are based on sandwich ELISA principles. One is bead-based array, and the other is solid-platform array. The bead-based array uses polystyrene beads and can be analyzed using a standard flow cytometer. While, the solid-platform-based array uses nanospot technology to spot cytokine/chemokine capture antibody onto either polyvinylidene fluoride (PVDF) membranes, nitrocellulose pads on the bottom of wells in a 96-well plate or on glass slides.
Fig.1 A schematic representation of a bead-based array
Fig.2 A schematic representation of a solid-platform array
Advantages of our Assays
Our multiplex cytokine and chemokine assays have the advantage to detect lots of cytokines/chemokines concurrently within the same experimental sample.
Well-incorporated with the cytometric bead array(CBA), Luminex xMAP, and FlowCytomix from Bender Medsystems, the bead-based array shows high sensitivity using different combinations of two or more different fluorescent dyes. Meanwhile, for the solid-platform-based array, the FASTQuant system and the SearchLight array system are two major advanced supports for the detection.
Ph.D-level scientists could design custom-oriented proposal for each specific case and give timely trouble-shooting to ensure the highest success of each project.
Creative Biolabs has the capability to enable you to free up your time for core work and project. Our service can be designed to meet your special needs if you have any requirements. If you are interested in our service, please feel free to contact us and our team will get back to you as soon as possible.
For any technical issues or products/services related questions, please leave your contacts as below, and our team will contact you at earliest convenience to let you know how we can be involved in your projects.
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