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CellRapeutics™ Multiplex Cytokine and Chemokine Assay

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Creative Biolabs has been an industry leader for a long time, and we have recently launched a proprietary multiplex cytokine and chemokine assay service to help customers detect different biomarkers in their samples.

Background Our Service WorkflowHighlights FAQs

Background

Cytokines are a class of polypeptide cell regulatory substances, including interleukins, growth factors, tumor necrosis factor, interferon, etc., which are mainly synthesized by peripheral immune cells. Cytokines can promote cell-to-cell interactions and have many biological functions, such as regulating growth, differentiation, inflammatory response, immune response, tumor growth and growth, etc.

Chemokines, also known as chemotactic cytokines, are a class of small molecule secreted proteins that bind to seven transmembrane G protein-coupled receptors and guide cell migration in steady state and inflammatory states. Cells expressing chemokine receptors migrate toward areas of high chemokine concentration. Chemokines and chemokine receptors play an important role in mediating cell migration, proliferation and defense against pathogen invasion, and are closely related to the occurrence and development of inflammation and cancer in the immune environment.

Fig.1 Different roles of cytokines and chemokines in tumor cells. (Propper, et al., 2022) Fig.1 Different roles of cytokines and chemokines in tumor cells.¹

Our Service

Based on the important role of cytokines and chemokines in cellular immunity, Creative Biolabs hereby introduces a proprietary multiplex cytokine and chemokine assay. This method helps you detect different cytokines and chemokines in different cell samples for research and clinical purposes.

  • CellRapeutics™ Multiplex Cytokine and Chemokine Assay

Unlike a single cytokine/chemokine assay, the CellRapeutics™ Multiplex Cytokine and Chemokine Assay can detect multiple biomarkers on a single sample simultaneously. The assay consists of two different technology platforms for the multiplexing of cytokines and chemokines. Both technology platforms are based on the sandwich ELISA principle. The difference is that one is a bead-based array and the other is a solid platform array. Magnetic bead-based arrays were analyzed using standard flow cytometry using polystyrene microspheres. Solid-state platform-based arrays dispense cytokine/chemokine capture antibodies to be detected onto polyvinylidene fluoride (PVDF) membranes, nitrocellulose pads at the bottom of wells of 96-well plates, or glass slides via nanodot technology.

  • Cell Type

Creative Biolabs has extensive experience and proficiency in the field of cell therapy, and we can provide but are not limited to cytokine/chemokine detection in the following cells:

Fig.2 Cell type of multiplex cytokine and chemokine assay. (Creative Biolabs Original) Fig.2 Cell type of multiplex cytokine and chemokine assay.

If you have any other special needs, you can contact us directly for further information.

Workflow

Fig.3 Magnetic bead array-based detection. (Naresh, et al., 2007)Fig.3 Magnetic bead array-based detection.²

Fig.4 Solid-state platform array-based detection. (Naresh, et al., 2007)Fig.4 Solid-state platform array-based detection.²

The above process is for our multiplex testing service platform, if you need to use other methods to test your sample. You can also contact us for more information.

Highlights

High-Throughput High Sensitivity Extensive Experience
The CellRapeutics™ multiplex cytokine and chemokine assay enables the simultaneous detection of different cytokines/chemokines in a single sample. The CellRapeutics™ multiplex cytokine and chemokine assay can be combined with different detection systems, such as Bender Medsystems' Flow Cytometry Array (CBA), Luminex xMAP, and FlowCytomix.
  • Chip-based can be combined with non-passable fluorescent dyes, thus showing high sensitivity.
  • Supports FASTQuant systems and SearchLight array systems.
  • Creative Biolabs scientists have extensive knowledge of cellular immunity and array arrays.
  • Solutions can be customized according to the specific needs.

Frequently Asked Questions

Q1: What is the process of the testing service?

A: First of all, our team conducts preliminary research and communication to determine the project plan and quotation. Then the customer sends us the samples to be tested, and after the test, we will deliver the samples and detailed test data/analysis report to the customer.

Creative Biolabs has the strength to provide customized services for your project. In addition to cytokine/chemokine assays, we also offer many other in vitro assays, you can click on the CAR Cell In Vitro Assay Service to see more.

References

  1. Propper, D.J.; et al. "Harnessing cytokines and chemokines for cancer therapy." Nat Rev Clin Oncol. (2022) 19, 237–253.
  2. Naresh. S.; et al. "Cytokine quantitation: Technologies and applications." Frontiers in Bioscience. (2007) 12(12):4682-95.
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