Gene Editing Service for CD8⁺ T Cell Metabolic Reprogramming

Empowering CD8⁺ T Cells Through Precision Metabolic Engineering

The field of adoptive cell therapy has witnessed a game-changing development: metabolic reprogramming is now revolutionizing how we boost CD8⁺ T cell performance. While conventional methods tweak receptor signals or add cytokines, we're finding that rewiring the cells' own metabolic machinery creates tougher, longer-lasting fighters - especially against tumors' immunosuppressive tactics. Gene editing technologies, particularly those enabling targeted and stable modifications, have become indispensable tools in fine-tuning these metabolic pathways. At Creative Biolabs, we offer comprehensive gene editing services to support metabolic optimization of CD8⁺ T cells for both research and therapeutic development.

Fig.1 Schematic diagram of knockout screening of primary CD8 T cells.¹

Customized Services for CD8⁺ T Cell Metabolic Enhancement

Our service portfolio is built to support a broad spectrum of CD8⁺ T cell metabolism studies. Whether the goal is to enhance glycolysis, mitochondrial fitness, or fatty acid metabolism, our gene editing solutions help clients achieve desired cellular traits. Services include:

• Metabolic Gene Knockout/Knockdown: Targeting negative regulators or metabolic checkpoints (e.g., PDHK1, ACAT1) to relieve functional constraints.
• Overexpression of Key Metabolic Genes: Introducing genes such as c-Myc, PGC1α, or Glut1 to boost anabolic or oxidative metabolism.
• Pathway-Specific Engineering: Modulation of glycolysis, fatty acid oxidation, glutaminolysis, or redox pathways.
• Reporter Line Generation: Creation of fluorescent or luminescent CD8⁺ T cells for real-time monitoring of metabolic activities.

All gene editing is performed in primary human or murine CD8⁺ T cells with rigorous functional validation post-modification.

Advanced Editing Platforms We Support

To accommodate diverse project requirements in CD8⁺ T cell metabolic reprogramming, Creative Biolabs employs a suite of precision editing platforms built upon engineered nucleases and transcriptional regulators. These platforms allow for efficient and targeted genomic manipulation with minimal off-target effects.

• Customizable Knockout/Knock-in Systems

We utilize highly adaptable editing tools capable of inducing gene disruption or precise sequence insertion at targeted genomic loci. These systems are optimized for robust performance in primary T cells and enable the modulation of genes involved in glycolysis, mitochondrial dynamics, fatty acid metabolism, and more.

• Transcriptional Modulation without Genomic Alteration

Our gene regulation platforms allow for reversible upregulation or suppression of metabolic genes through targeted binding to promoter regions. This non-destructive approach is ideal for studying gene function in a tunable and temporal manner.

• Nuclease-Free Editing Options

For sensitive applications where maintaining genome integrity is critical, we offer editing strategies that do not rely on direct DNA cleavage. These approaches support the modification of gene expression levels while avoiding permanent genomic changes.

• Flexible Delivery Vehicles

Depending on the nature of the target and cell type, we employ viral vectors or physical delivery methods (e.g., electroporation) to maximize efficiency, cell viability, and functional output in edited CD8⁺ T cells.

Step-by-Step Service Workflow

To streamline your research process, we've designed a transparent and customizable workflow:

Why Choose Creative Biolabs?

We are more than a gene editing service provider—we are your strategic partner in T cell metabolic research. What sets us apart:

Metabolism-Focused Expertise: Deep understanding of T cell bioenergetics and immunometabolism.

High Editing Efficiency: Proprietary electroporation and culture methods to maximize viability and edit rate.

Strict QC Standards: Rigorous quality control at each step to ensure reproducibility and functional relevance.

Flexible & Customizable: Services can be adapted to fit different disease models, experimental endpoints, and cell sources.

Contact Us

Our gene editing toolkit can reprogram T cell metabolism to boost your therapy's performance.

Let's make it happen:

Shoot us an email at ;

We'll schedule a 30-min consult to understand your exact needs;

Get a tailored solution designed for your specific research goals;

No canned proposals - just smart science made for your project.

Reference

1. Lin, Chun-Pu et al. "Multimodal stimulation screens reveal unique and shared genes limiting T cell fitness." Cancer cell vol. 42,4 (2024): 623-645.e10. Distributed under Open Access License CC BY 4.0, without modification.