What is a Luminex assay?

A luminex assay is a type of multiplexed immunoassay that allows for the simultaneous detection and quantification of multiple analytes (proteins, peptides, nucleic acids, etc.) in a single sample. It is based on the principle of xMAP (multi-analyte profiling) technology, which uses microspheres (beads) with unique fluorescent signatures to capture and detect specific analytes.

Can Luminex assay be applied in CAR-T cell therapy?

Luminex assays can be used in CAR-T cell therapy to monitor <a href='https://www.creative-biolabs.com/car-t/drug-discovery-assay-for-cytokine-storm-inhibition.htm'>cytokine release syndrome</a> (CRS) and assess the function of CAR-T cells. Luminex assays allow for the simultaneous monitoring of CAR-T cytokine profiles in a single sample, making them a valuable tool for evaluating the immune response. Additionally, Luminex assays can be used to measure the concentration of CAR-T cells in the patient's bloodstream, providing a non-invasive method for monitoring the effectiveness of the therapy.

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Luminex Assay Service for Monitoring CAR-T Cytokine Profiles

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Luminex Assay Service helps researchers to monitor the levels of multiple cytokines in a single sample simultaneously. It can be implemented in various applications that covering pathogen detection, drug discovery, biomarker discovery, vaccine development, and cancer research. In particular, Luminex Assay Service is useful for CAR-T development to monitor cytokine profiles of in vitro and in vivo experiment studies. Equipped with state-of-the-art facilities and the largest immediately available retail analyte selection system, Creative Biolabs is dedicated to providing customized Luminex assays services for our global customers.

What is Luminex Assay?

Luminex system and components.

Fig.1 Luminex system and components. (Dunbar, 2006)

The Luminex technology is a beads-based immunoassay that offers high-throughput detection of both protein and nucleic acid targets in multiple assay chemistries. Luminex assay, which allows for multiplex detection of up to 100 analytes simultaneously in one sample, can greatly reduce time, labor, and cost as compared to single-reaction-based detection methods.

The Luminex assay has been implemented in a variety of applications that range from transplant medicine, biomarker discovery and validation, pathogen detection, drug discovery, vaccine development, personalized medicine, neurodegeneration, and cancer research.

Luminex Assay Principle

General overview of the Luminex immunoassay detection scheme.

Fig.2 General overview of the Luminex immunoassay detection scheme.

Based on a suspension array, the platform contains capture moieties that are covalently coupled with dyed microspheres internally, also includes phycoerythrin-labeled antibodies binding to the specific antigen-antibody complex on the bead sets. Then, a Luminex instrument recognizes the response and analyses the difference in both bead sets, to identify the bead area and corresponding assigned analyte. The Luminex Assay system allows covalent coupling and detection of respective different types of molecules or macroparticles.

What can we provide?

Creative Biolabs provides customized Luminex assay service for multiple targets analysis, which allows you to carefully choose compatible targets and build your assay using analytes of optimized choice.

Creative Biolabs focuses on the following targets panels, but not limited to:

Human Cytokine/Chemokine
IL-1 alpha	IP-10/CXCL10	CD40 Ligand
IL-1 beta	MCP-1/CCL2	IFN-gamma
IL-1 ra/IL-1F3	MCP-3/CCL7	Eotaxin/CCL11
IL-2	MDC/CCL22	FGF basic
IL-4	MIP-1 alpha/CCL3	Flt-3 Ligand
IL-6	PDGF	Fractalkine/CX3CL1
IL-7	TGF-alpha	G-CSF
IL-8/CXCL8	TNF-alpha	GM-CSF
IL-10	TNF-beta/Lymphotoxin-alpha	Gro alpha/CXCL1
IL-17A	VEGF-A	Gro beta/CXCL2
......	......	......

Human Adipokine	Human Angiogenesis	Human Soluble Cytokine Receptor
Adiponectin	Angiopoietin-2	CD25/IL-2 R alpha
HGF	BMP-9	CD30
IL-1 beta	EGF	gp130
IL-6	Endoglin	IL-1 RI
IL-8/CXCL8	Endothelin-1	IL-1 RII
Leptin	FGF acidic	IL-4 R alpha
MCP-1/CCL2	FGF basic	IL-6 R alpha
beta-NGF	G-CSF	RAGE
Serpin E1/PAI-1	HB-EGF	TNF RI
……	……	……

Our Advantages

Quality-assured: Luminex assay services are performed under strict quality systems. We offer accurate, clearly presented, easy to interpret reports.
Availability: able to detect analytes across a large-scale of concentrations reliably.
High throughput multiplex analysis.
Require less sample volume (<50 µL).
Efficiency in terms of time and cost for multiple targets.
Flexible sample types choices.

Available samples for detection including human, mouse, and rat samples, which can be serum, plasma, and cell culture media types.

As an experienced customization service provider, Creative Biolabs can provide highly efficient and convenient strategies to satisfy your research demands, please feel free to contact us.

References

Purohit, S.; et al. Luminex and other multiplex high throughput technologies for the identification of, and host response to, environmental triggers of type 1 diabetes. Biomed Res Int. 2015.
Dunbar, S. A. Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid detection. Clin Chim Acta. 2006, 363(1-2):71-82.