NKT TCR-γδ T Cell-Specific Response Analysis Service

γδ T cells represent a potentially transformative advance in the treatment of malignant diseases. The development of gene-modified γδ T cells is a promising strategy in cancer immunotherapy. Aiming to fully support customers and advance the development of cell immunotherapy, Creative Biolabs provides comprehensive services to analyze and assess the function of NKT TCR-γδ T cells.

The approach of NKT TCR transfection into γδ T cells has a number of unique features that make it attractive for use as an immunotherapeutic approach to cancer treatment. The use of TCR-transfected γδ T cells could be a promising alternative for cancer immunotherapy.

• Not result in mispaired receptors
• Activate poorly responding γδ T cells
• Enhance the function of both innate and adaptive lymphocytes

NKT TCR engineered γδ T cells can recognize both the NKT cell and the γδ T cell ligands. After stimulation with ligands for αβ and γδ TCRs, NKT TCR-γδ T cells rapidly proliferate, produce cytokines and generate cytotoxicity against tumor cells. NKT TCR-γδ T cells show enhanced killing activity against the target cells. However, these cells have limited persistence in vivo, that be beneficial in attenuating cytokine release syndrome and neurotoxicity.

• γδ TCR enables major histocompatibility (MHC)-independent recognition of unprocessed antigen and recognizes 'phospho-antigens', such as isopentenyl pyrophosphate (IPP).
• iNKT cells recognize (glyco) lipids presented by CD1d, a non-polymorphic MHC-I-like molecule.

Fig.1 Modes of antigen recognition for CD1d-restricted T cell receptors. (Macho-Fernandez, 2015)

Our Services

To assess whether NKT TCR-γδ T cells respond to both the NKT/ γδ ligand, these cells are tested the proliferation and IFN-γ production after the phosphoantigen or α-GalCer stimulation as compared to control parental γδ T cells. Cytotoxicity assay is used to evaluate the antitumor effect by testing NKT TCR- γδ T cells against target cells that express phosphoantigen or α-GalCer.

At Creative Biolabs, our scientists and technicians can provide comprehensive assays to assess the NKT TCR-γδ T cell-specific response to both the NKT/ γδ ligand. Our experienced scientists and technicians can provide comprehensive assays including cytokine release assay, cytotoxic assay and cell proliferation assay. You can pick your targets of choice from our catalogue to set up your personalized panel.

Benefits of our services include:

• Proven performance
• End-to-end solutions
• Fast turn-around times
• Highly skilled team
• Scheduling flexibility

If you are interested in our services, please feel free to contact us and discuss your customized needs with our scientists.

Reference

1. Macho-Fernandez, Elodie, and Manfred Brigl. "The extended family of CD1d-restricted NKT cells: sifting through a mixed bag of TCRs, antigens, and functions." Frontiers in immunology 6 (2015): 362. Distributed under Open Access license CC BY 4.0, without modification.