CellRapeutics™ Soluble Ligand Sensing CAR Construction Service

Creative Biolabs has introduced the Soluble Ligand Sensing CAR, a novel CAR structure designed to respond to soluble ligands.

Soluble Ligand-sensing CAR: A Novel and Versatile Approach

A Soluble Ligand-Sensing Chimeric Antigen Receptor (CAR) is an engineered receptor expressed on the surface of T cells that specifically recognizes and binds to soluble ligands rather than cell surface antigens. This type of CAR is designed to target soluble proteins, such as cytokines, growth factors, or other signaling molecules that play a crucial role in disease pathogenesis. By leveraging the ability to target soluble ligands, soluble ligand-sensing CARs offer a novel and versatile approach for developing targeted immunotherapies across various medical fields.

CellRapeutics™ Soluble ligand-sensing CAR Construction Service at Creative Biolabs

Constructing a soluble ligand-sensing Chimeric Antigen Receptor (CAR) involves a series of steps from designing the CAR construct to expressing it in T cells. Creative Biolabs offers one-stop CAR-T cell development services, including vector construction and cell processing.

• CAR Design
  Creative Biolabs selects high-affinity binding domains specific to the target soluble ligand. Techniques such as directed evolution or rational design are used to enhance the stability, affinity, and specificity of the binding domain.
• CAR-T Gene Packaging & Delivery
  Creative Biolabs uses gene synthesis services to create DNA sequences encoding CAR constructs, including all designed domains. We cloned the synthesized CAR gene into the appropriate expression vector. Lentiviral or retroviral vectors are commonly used for stable integration and high expression of T cells.
• CAR Cell In Vitro Assay
  Creative Biolabs cultures and expands transduced T cells in the presence of cytokines such as IL-2 to obtain a sufficient number of cells for treatment. In addition, the ability of CAR-T cells to bind and respond to soluble ligands is tested using assays such as cytokine release assays, proliferation assays, and cytotoxicity assays.
• Safety Assessment
  Assess potential off-target effects and ensure the absence of replication-competent viral particles.

What We Can Offer

Creative Biolabs provides a comprehensive solution for the design and development of soluble ligand-sensing CARs. These innovative receptors extend the reach of conventional CAR technology by recognizing soluble factors such as cytokines, chemokines, or growth factors within the tumor microenvironment or diseased tissues. Mainstream technology platforms supporting this service include:

How to work

Our comprehensive service workflow is meticulously designed to efficiently and reliably move your universal CAR candidate from concept to preclinical validation.

Final Deliverables: Detailed technical report, engineered immune cell lines/cryopreserved CAR-expressing cells, and functional validation datasets (ligand specificity, signaling dynamics, safety evaluation).

Contact us to arrange a private consultation or obtain a comprehensive technical proposal.

Highlights of our Soluble Ligand-sensing CAR Construction Service

Applications of Soluble Ligand-sensing CAR

Soluble ligand-sensing CARs offer a range of innovative applications across various fields of medicine, particularly in oncology, autoimmune diseases, and beyond.

By leveraging the unique capabilities of soluble ligand-sensing CARs, these diverse applications can provide significant advancements in the treatment and management of a wide array of diseases.

Why Choose Creative Biolabs

As a qualified supplier of CAR construction, Creative Biolabs has the specific qualifications and capabilities to deliver high-quality, functional soluble ligand-sensing CAR constructs that meet research or therapeutic needs.

Frequently Asked Questions

Q1: When to consider a soluble ligand-sensing CAR construction?

A1: Constructing a soluble ligand-sensing CAR can be a strategic choice in various scenarios within the field of immunotherapy. Here are some key situations where this approach is particularly advantageous:

• Targeting soluble ligands in the tumor microenvironment;
• Autoimmune and inflammatory diseases;
• Hematological malignancies;
• Broadening the target repertoire;
• Enhancing safety profiles;
• Adaptive Response Modulation.

Q2: What are the precautions for soluble ligand sensing CAR?

A2:

• Target Selection: Choose soluble ligands that are critical in the disease pathology and are present in the disease microenvironment at significant levels.
• Affinity and Specificity: Ensure the ligand-binding domain has high affinity and specificity to avoid off-target effects and minimize potential toxicities.
• Safety: Design the CAR with safety mechanisms such as suicide genes or switchable CAR systems to control CAR-T cell activity and mitigate adverse effects.

Creative Biolabs can provide the necessary expertise and support for your specific needs in soluble ligand sensing CAR construction. If you have any needs for this field, please feel free to contact us. Our technicians in the relevant field will be happy to assist you.